Basic HTML Version
International Journal of Marine Science 2013, Vol.3, No.13, 105-110
http://ijms.sophiapublisher.com
109
Figure 3 Sampling location at Blanakan Ponds (left) and Marunda Ponds (right)
from the field location were wrapped with aluminium
foil and kept at –20
℃
until further analysis. Sampling
locations were determined using Global Positioning
System (GPS).
4.2 Extraction and fractionation
Samples of sediment and fish were freeze dried to
eliminate water content. Approximately 10 g dry
weight of sediment was weighted in a glass beaker.
Sample was added into the soxhlet apparatus and
surrogated with Naphtalene-D8 for recovery
assessment. The samples were extracted using 250 mL
(50:50 v/v) mixture of dichloromethane (DCM) and
hexane. After 18 hours extraction, the extracted
samples were evaporated under vacuum until 1 mL
using rotary evaporator. About 1 mL of extracted
sample was fractioned into subfraction using
silica-alumina column with 10 g of silica gel, 10 g of
alumina, and about 1 cm height of sodium sulphate
anhydrous. Hexane (50 mL) was eluted, pass through
into the silica-alumina column to elute aliphatic
(C
12
–C
34
) fraction followed by the 40 ml mixture of
DCM and hexane (50:50 v/v) to elute the PAHs fraction.
Internal standards of Acenaphthene D8 and
Penanthrene D8 were added, the samples were injected
to GC-MS (Prartono and Wolff, 1998; Elias et al.,
2007).
4.3 Gas chromatography-mass spectroscopy (GC-
MS) analysis
The PAHs fractions were analysed using GC-MS
model Shimadzu QP5000 with selective ion monitoring