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International Journal of Horticulture 2014, Vol.4, No.15, 1
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http://ijh.biopublisher.ca
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dissected using sterile blades and forceps under a
sterilized laminar hood chamber. After culturing, the
jars (containing the single node cuttings) were placed
in the growth chamber; the chamber was maintained at
22 ±2
℃
, 16 hours photoperiod and light intensity of
3000 lux.
After three weeks, the ten plantlets having grown from
the 10 single node cuttings were cut again and the
number of single nodes cuttings from each plantlet
was counted and recorded (subculture interval 1).
From each of the 10 plantlets, one single node cutting
was selected and cultured for three weeks in another
jar containing solid media. There were 10 such single
node cuttings in each jar. This was also done for the
two remaining jars to make three replicates. After
three weeks, the ten plantlets having grown from the
10 single node cuttings were cut again and the number
of single nodes cuttings from each plantlet was
counted and recorded (subculture interval 2). The
cycle was repeated again for another three weeks to
subculture 3.
Data analysis
The experiment was analysed as a split-plot design
where the potato cultivar was the main plot and the
subculturing interval (three of them) was treated as the
subplot. Data was analysed using Genstat statistical
package, 14
th
edition (Payne et al., 2011) and means
separated using Fisher’s Protected LSD Test (Steel
and Torrie, 1980).
Results and Discussion
There were significant differences (P≤0.001) in the
number of single node cuttings obtained among potato
cultivars, subculturing cycles and in the interaction
between potato cultivars and the subculturing cycle in
season 1 (Table 2).
Table 2 Skeletal ANOVA: Number of single node cuttings obtained from seven potato cultivars in three consecutive subculture
intervals in season 1
Source of variation
Degrees of freedom
MS
F.pr.
Rep
2
4.916
Potato cultivar
6
169.235
<.001
Error a
12
4.766
Subculturing cycle
2
185.949
<.001
Potato cultivar x subculturing cycle
12
11.227
<.001
Error b
28
2.421
Total
629
There was no significant difference in the number of
single node cuttings among potato cultivars between
subculture cycle 1 and 2 in the first season except for
Desiree (Figure 1). Generally, the third subculture
cycle gave higher number of single node cuttings than
the first two subculture cycles. In addition, Purple
Gold gave the least number of single node cuttings
(2.367) while Dutch Robyjn gave the highest (6.644).
In the second season, there were significant
differences (P≤0.05) in the number of single node
cuttings obtained among potato cultivars, subculturing
cycles and in the interaction between potato cultivars
and the subculturing cycle in season 2 (Table 3).
There was no significant difference in the number of
single node cuttings among potato cultivars between
subculture cycle 1 and 2 in the second season except
for Desiree (Figure 2). Generally, the third subculture
cycle gave higher number of single node cuttings than
Figure 1 Number of single node cuttings obtained from seven
potato cultivars over three consecutive subculture intervals in
season 1
Note: Within a potato cultivar, means sharing the same letter
are not significantly different from each other at P≤ 0.05. SC
1= subculture cycle 1, SC 2= subculture cycle 2, SC 3=
subculture cycle 3