International Journal of Horticulture, 2017, Vol.7, No.18, 146-153
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FD 69-1 on MPm2 medium. For cv. PRI-Red the response of Mpm4 and MPm5 was statistically non-significant.
While media MPm2 and Mpm4 were showing similar micropropagation response for genotype, FD 69-1. Like
wise the response to micropropagation of Mpm3 and MPm8 for both genotypes was also non-significant.
However the response of MPm2 for PRI-Red and MPm6 for FD 69-1 was similar (Table 3). From this study, it
was observed the number of shoots was being increased by increasing the concentration of BAP while lowering
the concentration of NAA. However the medium containing same concentration of BAP, and NAA inhibited
shoot induction and in this case greenish appearance was observed that could be depicted that might be there is
something, which was trying to promote shoot induction while at the same time other thing was inhibiting it
(Figure 3). For rooting these in vitro micropropagated shoots were then transferred to root induction medium
(Figure 1B). After root induction, plantlets were shifted to Belgium compost for acclimatization and hardening
(Figure 1C). In vitro micropropagated shoots of best micropropagation medium (MPm7) and genotype (PRI-Red)
whose combination and interaction yielded maximum number of shoots per explant were assessed for secondary
metabolites viz., alkaloids, total polyphenolic and flavonide contents in comparison to shoots of intact plant of
PRI-Red. Phytochemical analysis of shoots of intact plant and in vitro micropropagated shoots showed that
alkaloids were not detected in both
in vitro
micropropagated shoots and shoots of intact plant while both plants
showed positive test for presence of polyphenolics. Shoots of intact plant contained higher amount of total
polyphenolics (3.7 GAE mg/g fresh wt.) as compared to
in vitro
micropropagated shoots (2.8 GAE mg/g fresh
wt.). However, slightly higher amount of flavonide were detected in
in vitro
micropropagated shoots (1.8 mg/g)
than shoots of intact plant (1.6 mg/g) (Figure 5).
Table 2 Analysis of variance for in vitro micropropagation of
Solanum tuberosum
explant
Source of variation
Degrees of freedom Sum of squares
Mean squares
F-value
Genotype
1
44.467
44.467
225.15**
Media
7
135.540
19.363
98.04**
Genotype x Media
7
29.153
4.165
21.09**
Error
32
6.320
0.198
Total
47
215.480
Note: NS = Non-significant (P>0.05); * = Significant (P<0.05); ** = Highly significant (P<0.01)
Table 3 Media x genotypes interaction mean±SE: Mean values for shoot induction from explant of
Solanum tuberosum
Media
Genotypes
Mean
PRI-Red
FD 69-1
MP1
1.500±0.208efg
0.800±0.115e-h
1.150±0.189D
MP2
1.800±0.153de
0.200±0.058gh
1.000±0.365D
MP3
0.000±0.000h
0.000±0.000h
0.000±0.000E
MP4
3.600±0.404c
0.400±0.115fgh
2.000±0.740C
MP5
3.900±0.208bc
1.600±0.115ef
2.750±0.525BC
MP6
5.000±0.493b
1.800±0.153de
3.400±0.752B
MP7
7.500±0.611a
3.000±0.306cd
5.250±1.052A
MP8
0.000±0.000h
0.100±0.058h
0.050±0.034E
Mean
2.913±0.515A
0.988±0.211B
Note: Means sharing similar letter in a row or in a column are statistically non-significant (P>0.05). Lowercase letters represent
comparison among interaction means and uppercase letters were used for overall mean
