International Journal of Horticulture, 2017, Vol.7, No.18, 146-153
146
Research Article
Open Access
Assessing the Effect of Naphthalene Acetic Acid and 6-Benzylaminopurine on
In
Vitro
Micropropagation of Potato (
Solanum tuberosum
L
.
) and Estimation of
Secondary Metabolites in
In Vitro
Micropropagated Shoots
Siddra Ijaz
1
, Irfan Arshad
2
, Nazish Jahan
3
, and Imran-ul-Haq
4
1 Centre of Agricultural Biochemistry and Biotechnology/US-Pak Center for Advanced Studies in Agriculture and Food Security, University of Agriculture
Faisalabad, Pakistan
2 Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan
3 Department of chemistry, University of Agriculture Faisalabad, Pakistan
4 Department of Plant Pathology, University of Agriculture Faisalabad, Pakistan
Corresponding email:
International Journal of Horticulture, 2017, Vol. 7, No. 18 doi:
Received: 09 Jun., 2017
Accepted: 01 Jul., 2017
Published: 21 Jul., 2017
Copyright
©2017 Ijaz et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Ijaz S., Arshad I., Jahan N., and Imran-ul-Haq, 2017, Assessing the effect of naphthalene acetic acid and 6-Benzylaminopurine on
in vitro
micropropagation of
potato (
Solanum tuberosum
L.) and estimation of secondary metabolites in
in vitro
micropropagated shoots, International Journal of Horticulture, 7(18):
146-153 (doi:
)
Abstract
The effect of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP) was investigated on in vitro
micropropagation of potato (
Solanum tuberosum
L.) and secondary metabolites of
in vitro
micropropagated shoots were also
estimated. Eight micropropagation media containing different level of NAA and BAP were studied and the effect of NAA and BAP
on in vitro micropropagated shoots was assessed. However, in this research study, medium MPm 7 having 2.75 mg/l BAP was
proved to be best for giving maximum number of shoots per explant in both genotypes to be studied. The genotype, PRI-Red yielded
maximum number of shoots on this medium thus these in vitro micropropagated shoots were subjected to phytochemical analyses
and were compared with shoots of intact plant (field grown) for secondary metabolites viz., alkaloids, flavonide and total
polyphenolic contents. In phytochemical analyses, no alkaloids were not detected in both
in vitro
micropropagated shoots and shoots
of intact plant while both plants showed positive test for presence of polyphenolics. Shoots of intact plant contained higher amount
of total polyphenolics (3.7 GAE mg/g fresh wt.) as compared to
in vitro
micropropagated shoots (2.8 GAE mg/g fresh wt.). However,
slightly higher amount of flavonide were detected in
in vitro
micropropagated shoots (1.8 mg/g) than shoots of intact plant (1.6
mg/g).
Keywords
Potato; Polyphenoles; Micropropagation; 6-Benzylaminopurine; Naphthalene acetic acid
1 Introduction
Potato (
Solanum tuberosum
L.) is one of the most important vegetable crop, belongs to an economically
important family Solanaceae and is grown worldwide. In some countries like Bangladesh, it secured second
position as a staple food crop after rice (Parveen et al.
,
2014). Due to its high nutritious value, the potato crop has
gained much importance. It is considered as the third most important crop of the world after wheat and rice and is
consumed by more than one billion people worldwide (Venkatasalam et al.
,
2013). In Pakistan, it was grown on
172 thousand hectares during the year 2012 with the production of 3767.2 thousand tons which was 11.0 % more
than last year (Economic survey of Pakistan, 2013). In Pakistan, the production rate of the potato crop is very low
compared to other countries due to the poor-quality seed.
Conventionally the potato crop is propagated vegetatively by seed tubers which give uniform growth and yield.
The problem of deterioration of crop is faced due to virus infection and degree of deterioration changes from one
crop to other and also to cropping seasons. The transmission of viruses is due to the planting of infected tubers.
The prevalence of virus infection can reach up to 100% in successive crop seasons and can reduce the yield up to
half or one third. This is the major problem faced by seed tuber producers.
As discussed above the potato crop is usually propagated asexually by using tubers. The vegetative propagation
in potato can also be achieved through tissue culture which is a technique of great potential in the field of
