International Journal of Aquaculture, 2016, Vol.6, No.8 1-10
1
Research Article Open Access
Micropropagation and
in vitro
flowering of an ornamental aquarium plant
Lindernia antipoda
(L.) Alston
Jabir T., Sheeja George., Anjana Raj., Sree Lakshmi S., Aneykutty Joseph .
Department of Marine Biology, Microbiology & Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kerala, India
Corresponding author Email
International Journal of Aquaculture, 2016, Vol. 6, No.8 doi
Received: 29 Feb., 2016
Accepted: 12 Apr., 2016
Published: 26 Apr., 2016
Copyright © 2016
Jabir et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Jabir T., Sheeja George., Anjana Raj., Sree Lakshmi S., Aneykutty Joseph.
, 2016, Micropropagation and
in vitro
flowering of an ornamental
aquarium plant
Lindernia antipoda
(L.) Alston, 6(8): 1-10 (doi
Abstract
Lindernia antipoda
(L.) Alston is one of the valuable aquatic ornamental plants which have a bright potential in the
aquarium trade. In the present study, a protocol for rapid shoot multiplication and
in vitro
flowering from axillary bud was developed.
Half MS media with 1 mg/L benzylaminopurine (BAP) was the found to be the best medium for culture initiation and establishment.
The effective medium for shoot multiplication was MS+1 mg/L BAP+0.2 mg/L NAA. In this media, 85% of the cultures exhibited
multiple shoot proliferation. The highest shoot proliferation and shoot length were also acquired in this media. Plant showed
highest in vitro flowering in MS medium supplemented 1 mg/L BAP and 0.2 mg/L α-Naphthalene acetic acid (NAA). All
concentrations of NAA induced rooting. Hardening of
in vitro
cultured plantlets were attained through polythene propagator system
and 90% of the plants survived, and it was successfully transformed in to an aquarium.
Keywords
Lindernia antipoda
; Aquarium plant; Micropropagation;
in vitro
flowering
1 Introduction
Lindernea antipoda
(L.) Alston is an evergreen amphibian herbaceous plant belongs to the family Linderniaceae,
found in Tropical Asia. It is a large and taxonomically difficult genus; most species are found in swamps and also
found in temporarily submerged areas. They are mainly used for filtration and cleaning of water in canals and
lakes. Some of the species belonging to the genus
Lindernia
are used as ornamental aquarium plants.
L. antipoda
(L.) produces small white flowers and bright green leaves and is widely used as an aquarium plant. Native range
of
Lindernea
sp. is tropical and subtropical Asia from India and Sri Lanka, throughout Malaysia and eastward to
northern Australia; apparently adventives further eastward (Smith, 1992). The systematic position of
L. antipoda
(L.) Alston is as follows.
Kingdom: Plantae
Division: Tracheophyta
Class: Magnoliopsida
Order: Lamiales
Family: Linderniaceae
Natural propagation of aquatic plant species is limited due to the production of small number of plants with a long
cultivation period, disease and requirement of large space for propagation (Sulaiman, 2004).
In vitro
propagation
of aquarium plants can solve many of these problems such as unreliable supply, over collection, variable plant
quality and frequent losses from poorly characterized water transmitted diseases and destruction of endangered
species (Kane et al., 1999; Sarasan et al., 2006). Hence, conservation and commercial production of
Lindernia
sp.
in order to meet the market demand can be achieved
via
the development of efficient and economically viable
micropropagation protocols.
Moreover, the collection of plant materials from the wild resources can be reduced or prevented with the
production of clonal propagated plants. Previous studies have reported the
in vitro
propagation protocols for some
