International Journal of Aquaculture, 2016, Vol.6, No.8, 1-10
8
Liquid shake cultures are proven extremely successful in plant tissue culture propagation (Tewary and Oka, 1999;
Han et al., 2004; Kadota and Niimi, 2004). According to Piatczak et al. (2005), the fast proliferation of shoots in
liquid medium is due to the fact that the shoots were totally immersed in the liquid medium presenting a large
surface area for the uptake of nutrients and plant growth regulators. Higher shoot proliferation in liquid medium
was also reported for
Dioscorea japonica
(Kadota and Nimii, 2004).
Flowers were initiated on MS medium with all combinations of cytokinin (BAP) and auxin (NAA), however the
flowering shoots were found on combination of 1 mg/l BAP with 0.2 mg/l NAA.(Table 5 and Figure 4). Mostly
BAP has been used to induce
in vitro
flowering (Wang et al., 2002; Saritha and Naidu, 2007; Jana and Shekhawat,
2011). Phytohormones play a major role in the process of flowering by bringing about changes such as initiation
of mitosis and regeneration of cell division and organ formation by cytokinin (Tylor et al., 2005; Rathore et al.,
2014). Synergic combination of both auxin and cytokinin was found finest for flowering. Combination of auxin
and cytokinin was alone used to induce flowering in
Withania somnifera
(Saritha and Naidu, 2007) and
Cleome
viscosa
(Rathore N.S. et al., 2014). All concentrations of NAA possess rooting after flowering.
L. antipoda
(L.)
was amphibious types of aquatic plants; hence polythene propagator system was recommended (Yapabandara et
al., 2006). 100% survival rate was obtained in polythene propagator system. Then the plants were successfully
transplanted in to aquarium.
Figure 4:
in vitro
flowering of
L. antipoda
(L.) on BM
3
media 4 weeks after culture initiation
In conclusion, in the present work we successfully developed a suitable micropropagation protocol for large scale
multiplication of aquarium plants
L. antiopoda
(L.) Alston. The best medium for shoots multiplication and root
proliferation was MS media supplemented 1mg/l BAP and 0.2mg/l NAA. Shoot multiplication and
in vitro
flowering was established in MS media supplemented with 1mg/l BAP & 0.2 mg/l NAA (BM
3
). The results of the
study delineate successful
in vitro
proliferation of aquarium ornamental plant
L. antiopoda
(L.) Alston.
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