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International Journal of Aquaculture, 2014, Vol.4, No.05
http://ija.sophiapublisher.com
31
micro- and macro- coronary diseases).
IA = [(4
×
C14:0)+C16:0+C18:0] / (
∑
MUFA+
∑
PUFA-n6+
∑
PUFA-n3)
2) Index of thrombogenicity (IT): showing the
tendency to form clots in the blood vessels. This is
defined as the relationship between the pro-
thrombogenetic
(saturated)
and
the
anti-
thrombogenetic fatty acids (MU-FAs, PUFAs – n6
and PUFAs – n3) (Garaffo et al., 2011; Kaya and
Turan, 2008).
The following equation was applied:
IT = (C14:0+C16:0+C18:0) / (0.5MUFA+0.5PUFA-n6
+3PUFA-n3+PUFA-n3/PUFA-n6)
1.5 Statistical Analysis
Data were subjected to analysis of variance (ANOVA)
and mean comparison was carried out using Duncan’s
test. Statistical analysis was performed with SPSS.
Significance was established at P<0.05.
2 Results
2.1 Fatty acid profile
The fatty acid composition of goatfish and pony fish
are shown in Table 1 and Figure 1.
Table 1 Fatty acid profile (%) of
L. bindus
and
U. sulphureus
in Fall and Spring
Leiognathus bindus
Upeneus sulphureus
Fatty acid
Fall
Spring
Fall
Spring
(12:0)
0.1±0.02
ND
0.25±0.01
ND
(14:0)
5.49±0.01
5.95±0.00
2.9±0.03
2.45±0.01
(15:0)
0.47±0.00
ND
0.34±0.00
ND
(16:0)
29.57±0.03
33.97±0.01
27.49±0.02
39.7±0.05
(17:0)
1.04±0.02
1.36±0.00
0.92±0.05
1.76±0.02
(18:0)
10.75±0.02
10.25±0.02
16.41±0.03
11.97±0.03
(20:0)
0.15±0.03
1.78±0.02
1.97±0.01
ND
(21:0)
0.43±0.00
ND
0.53±0.00
ND
(22:0)
0.6±0.01
1.06±0.04
ND
1.8±0.00
(23:0)
0.084±0.01
ND
ND
ND
(24:0)
1.32±0.02
ND
1.59±0.02
ND
(14:1)
1.57±0.10
1.08±0.02
1.36±0.04
0.89±0.02
(16:1)
9.59±0.05
13±0.01
5.27±0.03
6.97±0.03
(18:1)
12.09±0.01
18.4±0.00
15.40±0.09
24.45±0.06
(20:1)
0.71±0.01
1.11±0.03
0.83±0.03
ND
(22:1)
0.080±0.03
ND
0.35±0.02
ND
(24:1) ω9
0.87±0.02
ND
0.29±0.00
ND
(18:2) ω6
0.66±0.03
0.8±0.03
1.12±0.03
0.85±0.00
(20:2) ω6
0.29±0.05
ND
0.25±0.01
ND
(18:3) ω3
0.36±0.05
0.61±0.06
0.29±0.00
0.33±0.01
(18:3) ω3
1.07±0.00
0.47±0.01
1.54±0.01
0.5±0.00
(20:3) ω6
0.27±0.02
ND
0.23±0.01
ND
(20:4) ω6
2.99±0.01
ND
4.58±0.02
ND
(22:5) ω3
ND
1.66±0.00
1.74±0.01
0.46±0.00
EPA ω3
5.7±0.01
4.63±0.02
4.65±0.03
2.43±0.01
DHA ω3
3.35±0.02
3.78±0.04
4.5±0.00
4.33±0.02
SFA
50.004±0.02
54.37±0.02
52.4±0.12
57.68±0.05
MUFA
24.919±0.03
33.59±0.00
23.5±0.03
33.31±0.01
PUFA
17.27±0.01
11.95±0.03
18.9±0.04
8.9±0.02
HUFA
14.62±0.02
10.07±0.01
15.47±0.02
7.22±0.05
Note: n= 3 ± standard error; SFA: saturated fatty acid, MUFA: mono unsaturated fatty acid, PUA: poly unsaturated fatty acid, HUFA:
high unsaturated fatty acid; ND: Non-detected