Page 8 - IJA 1012-Vol.3 No.24

International Journal of Aquaculture, 2013, Vol.3, No.24, 138- 146
http://ija.sophiapublisher.com
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Table 2 List of two Cyprinidae species sampled for DNA extraction
S. No Species name Sample code Location
1 Cyprinus carpio communis Sc-1 to Sc- 5 River Jhelum, Srinagar
2 Cyprinus carpio communis Sc-6 to Sc- 10 Telbal Nallah, Srinagar
3 Cyprinus carpio communis Sc-11 to Sc- 15 Dal lake, Srinagar
4 Cyprinus carpio communis Sc-16 to Sc-20 Anchar lake, Srinagar
5 Cyprinus carpio communis Sc-21 to Sc-25 River Jhelum, Sopore
6 Cyprinus carpiospecularis Mc-1 to Mc- 5 River Jhelum, Srinagar
7 Cyprinus carpio specularis Mc-6 to Mc- 10 Telbal Nallah, Srinagar
8 Cyprinus carpio specularis Mc-11 to Mc- 15 Dal lake, Srinagar
9 Cyprinus carpio specularis Mc-16 to Mc-20 Anchar lake, Srinagar
10 Cyprinus carpio specularis Mc-21 to Mc-25 River Jhelum, Sopore
11 Carassius carassius Cc-1 to Cc- 5 River Jhelum, Srinagar
12 Carassius carassius Cc-6 to Cc- 10 Telbal Nallah, Srinagar
13 Carassius carassius Cc-11 to Cc- 15 Dal lake, Srinagar
14 Carassius carassius Cc-16 to Cc-20 Anchar lake, Srinagar
15 Carassius carassius Cc-21 to Cc-25 River Jhelum, Sopore
Table 3 Comparison of genetic diversity and dissimilarity coefficients among 75 individuals of Cyprinidae species by PCR-RFLP
Restriction Enzyme Type Total number of bands Percentage polymorphism Shannon index (H’)
MboI Tetra-cutter 10.0 50.0 0.29
Hin6I Tetra-cutter 7.0 72.7 0.23
BsuRI Tetra-cutter 7.0 87.5 0.23
MspI Tetra-cutter 8.0 87.5 0.25
Total score 32.0
Mean per enzyme 8.0 74.4 0.58
Sc Mc C c
Figure 3 Restriction digestion profiles of Cyt b gene by MboI in two Cyprinidae species. Sc is Cyprinus carpio communis, Mc
Cyprinus carpio specularis and Cc. Carassius carassius
The band size varied from 100 to 600 bp. The average
number of bands per enzyme ranged between 8 (MspI)
and 10 (MboI) with a mean of 8.0. The proportion of
polymorphic markers across the enzymes ranged from
50% to 87.5% with an average of 74.4%. Restriction
digestion with the above four enzymes showed that
the total size of the bands was always near to the
expected size (680 bp). The replication digestion was
performed with each of the screened restriction
enzymes for at least two times to check the
reproducibility. A total of 2-4 polymorphic restriction
fragments were observed in each of the species with
each enzyme. Restriction enzyme MboI and BsuRI
efficiently generates fragments in all the species. The
size in the restriction fragment with MboI varied from
155 bp to 300 bp in C.c. communis, 174 bp to 300 bp
in C.c. specularis and 167 bp to 300 bp in C.
carassius. Also, the size in the restriction fragment