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International Journal of Aquaculture, 2013, Vol.3, No.24, 138
-
146
http://ija.sophiapublisher.com
140
Sc Mc Cc
Figure 1 RAPD patterns of 75 specimens of two Cyprinidae species; Sc (1-25) is
Cyprinus carpio
var.
communis
, Mc (1-25) is
Cyprinus carpio
var.
specularis
and Cc (1-25) is
Carassius carassius
produced by primer S-161, M is 1 kb ladder (Gene Rular
TM
100 bp plus) and N is negative control
polymorphic and non-polymorphic band using Karl
Pearson’s formula.
From clustering analysis UPGMA dendrogram revealed
that crucian carp distributed in III cluster displayed
maximum dissimilarity coefficient of 0.49 with other
individuals, while, other two species i.e. Scale carp
and Mirror carp clearly distributed in two clusters I
and II respectively as shown in Figure 2. The RAPD
analysis proved to be an effective and efficacious
technique to measure the magnitude of diversity and
polymorphism of the species.
Of the seven restriction endonucleases used, four
restriction endonuclease enzymes viz.,
MboI, BsuI,
MspI, Hin6I (Fermentas, Life Sciences) were found
efficiently producing polymorphic patterns in Cytb
region (Table 3 and Figure 3). A total of 32 restriction
bands were observed with these enzymes.
Figure 2 Comparison of two dendrograms obtained with RAPD and PCR-RFLP analyzed for 75 individuals of two Cyprinidae
species. See Table 2 for specimen codes