Genomics and Applied Biology 2018, Vol.9, No.9, 56-61
56
Research Report
Open Access
Immunohistochemical Research of the Expression of Nile Tilapia (
Oreochromis
niloticus
) NCCRP-1 in Various Tissues
Zheng Qi
1,2,3
, Huang Yu
1,2,3
, Yuan Man
1
, Cai Jia
1,2,3
, Tang Jufen
1,2,3
, Lu Yishan
1,2,3
, Wu Zaohe
1,2,3
, Jian Jichang
1,2,3
1 Fisheries College of Guangdong Ocean University, Zhanjiang, 524088, China
2 Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals, Zhanjiang, 524088, China
3 Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, 524088, China
Corresponding author email:
Genomics and Applied Biology, 2018, Vol.9, No.9 doi:
Received: 16 Oct., 2018
Accepted: 05 Nov., 2018
Published: 29 Dec., 2018
Copyright © 2018
Zheng et al., This article was first published in Genomics and Applied Biology (2018, 06: 2356-2360) in Chinese, and here was
authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Zheng Q., Huang Y., Yuan M., Cai J., Tang J.F., Lu Y.S., Wu Z.H., and Jian J.C., 2018, Immunohistochemical research of the expression of Nile tilapia
(
Oreochromis niloticus
) NCCRP-1 in various tissues, Genomics and Applied Biology, 9(9): 56-61 (doi:
Abstract
It is well known that non-specific cytotoxic cell is a kind of lymphocyte in teleosts, which plays an important role in
nonspecific immunity by up-regulating inflammation response and releasing cytokines. NCCs are equivalents of NK cells in
mammals. NCC in fishes distributes in tissues and organs with spontaneous cytotoxicity which can kill various target cells such as
allogenic or heterogeneous tumor cells, parasitic protozoan and cells induced by pathogens. NCCRP-1 is a landmark receptor on
NCCs. This study sectioned brain, head kidney, liver and spleen of Nile Tilapia (
Oreochromis niloticus
) and stained NCCRP-1 as
well. The study obtained the distribution of NCCs in tissues via immunohistochemical analysis. Furthermore, we also isolated and
obtained NCC of head kidney through Percoll density gradient centrifugation, stained by immunohistochemistry and tagged with
FITC. The distribution was the same as the staining result in tissues. This study observed the distribution characteristics of NCC in
tissues more clearly than qPCR technique, which might lay the foundation for further studies on the function of NCC.
Keywords
Nile tilapia (
Oreochromis niloticus
); NCCRP-1; Immunohistochemistry
Background
Non-specific cytotoxic cell (NCC) is a kind of lymphocyte in teleost fish, which can induce nonspecific immune
response in fish under adverse environment or pathogen stimulation, and its effect is equivalent to the NK (natural
killer cell) cell in mammals (Jaso-Friedmann et al., 2004). The effector target cells of NCC include tumor cells
and parasitic protozoa, which indirectly resist virus invasion by killing viral-infected abnormal cells (Graves et al.,
1985). In addition, through cytokine regulation, fish NCC can also cause inflammatory reactions like NK cells in
mammals (Jaso-Friedmann et al., 2001). At present, NCC has been confirmed to exist in many organs or tissues of
teleost fishes, including the head-kidney (Mulero et al., 1994), spleen (Wood and Cheers, 1985), liver (Barnes et
al., 1977) and peripheral blood (Seppola et al., 2007).
NCCRP-1 is a marker receptor on NCC. Studies by Jaso-Friedmann et al. (2004) predicted that NCCRP-1 might
have three signal domains: antibody binding domain, signal transduction domain and transcription activation
domain. The existing reports about NCCRP-1 were concentrated on
Danio rerio
(Reimers et al., 2006),
Ictalurus
punctatus
(Evans et al., 1996),
Oncorhynchus mykiss
(Fischer et al., 2013),
Cyprinus carpio
L. (Seppola et al.,
2007) and
Stegastes partitus
(McKinney and Schmale, 1997). NCCRP-1 is considered to be able to recognize and
bind various target cells to induce cytotoxic reactions.
Tilapia is an important aquatic economic animal, which can provide high quality animal protein. Because of its
delicious flavor with less thorn, more meat and strong adaptability, tilapia has been widely cultured in southern
China and has brought considerable output value (Li, 2006). In recent years, environmental pollution and poor
management patterns had led to large-scale outbreaks of parasites, bacteria and viruses, resulting in serious
economic losses (Gan et al., 2014). Immunohistochemistry is a basic experimental method in human clinical
tumor research (Li et al., 2014). Furthermore, this experimental method also plays a certain role in fish research
(Yang et al., 2017), and specific substances are shown through nonspecific antibody binding and staining.
