Genomics and Applied Biology 2018, Vol.9, No.3, 14-19
18
According to the data, there were some differences between two genes under different abiotic stresses. In addition,
the expression of genes in different tissues was also different. Under the salt stress, gene expression was
significantly different, which is consistent with (Shi et al., 2013) conclusion. They believe that the mutant salinity
is due to the excessive synthesis of NO and polyamine. This is probably because that arginase competes with other
enzymes that degrade arginine, and strength of enzyme activity determines the direction of arginine
decomposition (Yang and Gao, 2007).
Figure 5 Expression analysis of arginase gene in different tissues under different concentration (A) and different times (B) of
Arginine stress
3 Conclusion
This study mainly focused on the expression analysis of arginase genes in respons to different abiotic stress during
the early-seed germination and seedling growth stage using real-time quantitative PCR. The expression of
AtArgAH1
and
AtArgAH2
was highly expressed in root and siliques under normal conditions, however,
AtArgAH2
was higher expression than
AtArgAH1
in mature stage. In salt stress, the expression levels of
ArgAH1
and
ArgAH2
was up-regulated in both roots and cotyledons. But the expression of
AtArgAH2
relative to
AtArgAH1
is more
significant. Under the NH
4
Cl stress,
AtArgAH1
gene was up-regulated at higher concentrations. Under the urea
stress, differences in the expression of two genes were large and irregular. Under the arginine stress, difference
was not obvious.
Autho
r
s’ contributions
Y. Bu and S. Liu designed the study. X. Shen performed the experiments and analyzed the data. Y. Bu, S. Liu and T. Takano
supervised the study and critically revised the manuscript. All authors read and approved the final manuscript.
This work was supported by the Special Fund for Forest Scientific Research in the Public Welfare (201404220), and Program for
Changjiang Scholars and Innovative Research Team in University (No. IRT13053) awarded to Shenkui Liu. Further supported by the
National Natural Science Foundation (NSFC) of China (No. 31500501) and Fundamental Research Funds for the Central
Universities (2572016CA14) awarded to Yuanyuan Bu.
References
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PMid: 18425591
Chen H., McCaig B. C., Melotto M., He S.Y., and Howe G.A., 2004, Regulation of plant arginase by wounding, Jasmonate, and the Phytotoxin Coronatine,
Journal of Biological Chemistry, 279(44): 45998-46007
PMid: 15322128
