Genomics and Applied Biology 2016, Vol.7, No.5, 1-8
7
at least two groups with virus-cell junction at DR1 and DR2 downstream through the core gene or upstream
through the X gene, this may related to the replication and integration function of HBV DR region. Since DRs are
5’ ends of the minus and plus DNA strands of the linearized HBV genome, it is reasonable to argue that the
frequent use of the DRs as integration sites is likely due to the preferred use of the free ends of replication
intermediates of HBV. Although fusions can occur close to both DR1 and DR2, the viral human fusion transcripts
were strongly biased to regions near DR1. But it is still known little by us how the gap is filled up to form
cccDNA, whether the gap filled up by recombinant PCR separated again when the DNA fragment integrated into
host genome that we don’t know. So whether the four groups included in the HepG2 cells, it needs to further
research. This DNA fragment that we isolated has a significance to find out the mechanism of how the gap is
filled up and it is very important to make it clear that what is the relationship between the DR region and the
integration of HBV. By researching this HBV DR region, maybe we can know more about the formation
mechanism of HBV cccDNA, Furthermore, we can know more about the replication and integration mechanism
of HBV DNA. In addition, researching this DNA fragment maybe has great significance for preventing the
replication of HBV.
5 Conclusions
The HBV Creg fragment can integrate into HepG2 cells genome. And the integration of HBV is related to DR
region, the way of HBV Creg DNA fragment integrated into HepG2 cells is from DR region to both sides.
Competing interest
The authors declare that they have no competing interests.
Compliance with ethics guidelines
This article does not contain any studies with human or animal subjects performed by any of the authors.
Author contributions
Haoxiang Luo conceived the study, performed the experiments, and wrote the paper. Long Gu and Lihong He participated in the
study design, experiments and literatures. All authors read and approved the final manuscript.
Acknowledgments
This work was supported by the Science and Technology Department of Guizhou Province of China (No. J [2013]2268).
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