Bioscience Methods 2015, Vol.6, No.3, 1-9
3
Statistical analysis of the experiments was performed using
the Handbook of Biological Statistics (McDonald, 2008).
3 Results
3.1 Compatibility of
Trichoderma
species against
fungicides
Species of
Trichoderma viz., T. viride, T. harzianum, T.
koningii, T. pseudokoningii
and
T. virens
were isolated
from the rhizosphere soil of different crop plants.
These isolates were deposited in Research laboratory,
Department of Botany, Arts Science and Commerce
College Naldurg. These isolates were maintained on
PDA (Potato Dextrose Agar) media slants. Species of
Trichoderma
were amended with fungicides and
results obtained following (Table 1&2; Figure 1).
Table 1 Compatibility of
Trichoderma
spp with different concentrations of Mancozeb fungicide.
Sr. No.
Trichoderma
spp
Control (mm)
Radial growth in different concentration (μg/ml)
1000
2000
3000
4000
5000
6000
7000
8000
1
T. viride
90
48
(55)
35
(37)
34
(36)
27
(29)
14
(15)
08
(09)
03*
(05)
00
(00)
2
T. harzianum
89
72
(83)
63
(69)
57
(66)
49
(58)
40
(47)
32
(33)
19
(20)
00*
(00)
3
T. koningii
90
47
(53)
41
(43)
36
(40)
33
(35)
27
(31)
21
(27)
12
(14)
00*
(00)
4
T. pseudokoningii
90
31
(33)
24
(27)
18
(19)
09
(11)
00*
(00)
00
(00)
00
(00)
00
(00)
5
T. virens
88
82
(86)
74
(83)
63
(72)
51
(56)
40
(45)
22
(23)
13
(15)
00*
(00)
Note: *Significantly reduced the radial growth of mycelium.
Figures in parentheses are arcsine transformed value of radial growth of mycelium.
Table 2 Compatibility of
Trichoderma
spp with different concentrations of Captan fungicide.
Sr. No.
Trichoderma
spp
Control (mm)
Radial growth in different concentration (μg/ml)
100
200
300
400
500
600
700
1
T. viride
90
53
(47)
38
(41)
28
(33)
13
(14)
04
(04)
02*
(03)
00
(00)
2
T. harzianum
89
57
(62)
37
(38)
24
(25)
11
(12)
06
(06)
03*
(04)
00
(00)
3
T. koningii
90
48
(51)
35
(37)
25
(29)
12
(14)
05
(07)
02*
(02)
00
(00)
4
T. pseudokoningii
90
34
(35)
28
(30)
21
(24)
08
(11)
00*
(00)
00
(00)
00
(00)
5
T. virens
89
62
(70)
47
(54)
28
(29)
09
(10)
00*
(00)
00
(00)
00
(00)
Note: *Significantly reduced the radial growth of mycelium.
Figures in parentheses are arcsine transformed value of radial growth of mycelium.
a.
Trichoderma viride
:
Trichoderma. viride
showed
growth on medium containing Mancozeb@1000,
2000, 3000, 4000 & 5000 μg/ml. The growth of
T.
viride
is least on media containing Mancozeb@6000
and 7000 μg/ml, but no growth was observed when
Mancozeb was used @8000 μg/ml.
Trichoderma
viride
grow easily when Captan was used @ 100,
200, 300 and 400 μg/ml, the growth was minimum
on Captan@500 and 600 μg/ml, but no growth were
recorded on Captan@700 μg/ml.
b.
Trichoderma harzianum
:
Trichoderma harzianum
grow
at lower concentration of Mancozeb. The growth was
inhibited when concentration of Mancozeb was more
than 8000 μg/ml. However, increased concentration of
Mancozeb decreased the radial growth. In media
containing Captan@700 μg/ml, no growth was recorded.
The growth of
T. harzianum
was observed at lower
concentration from 100 to 400μg/ml.
c.
Trichoderma koningii
:
Trichoderma koningii
showed
growth inMancozeb@1000 to 5000 μg/ml. The growth
