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Tree Genetics and Molecular Breeding 2014, Vol.4, No.2, 1
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Table 1 Newly designed SSR markers on LG16
Marker
Primer sequence F-R
Source contig
Contig position
Amplic
on (bp)
z
SSR
z
Map
position
LG16-769020-1 F- CATCAGATGATTATCCCTTG
ACTAC
MDC013096.127 chr16:769020..8161
47
115
(AT)
15
LG16
R- TGATCGGAAACAGGACCACT
LG16-769020-2 F- GGACGCGAACACATGCTTAT MDC013096.127 chr16:769020..8161
47
297
(AT)
12
LG16
R- GTCCTTTTCATACCGCTGGA
LG16-837763
F- TCAGGCCACAAACAAACAAA MDC013096.125 chr16:837763..8967
6
150
(AG)
25
LG16
R- TTCTCTGCCAAACCCCTTT
LG16-1003247-1 F- CCTCAACTGCACAAAGCAAA MDC006875.277 chr16:1003247..105
2856
149
(AC)
18
LG16
R- CGTCATCAACAGGATGCAGA
LG16-1003247-2 F- TAGAGGCAAACCGACGGTAG MDC006875.277 chr16:1003247..105
2856
142
(AG)
14
not
determined
R- CCTTTCCTTTCCCTCCAAAG
LG16-1140516
F- GCAGGAAACTTCAGCAAACA MDC010932.700 chr16:1140516..115
6076
393
(AG)
24
not
determined
R- GTGAAGATCACGCGGTTGAG
LG16-1159074
F- TTCTCTGCCAAACCCCTTTT MDC010932.632 chr16:1159074..116
3563
136
(CT)
17
LG16
R- ACATTCCGCCACACTACCTC
LG16-1199876
F- GCGATCGGTTCCGTTCTA
MDC010344.263 chr16:1199876..124
3332
135
(TC)
21
LG16
R- GTGAGCCGGACTTTTGGTTA
LG16-1352468
F- AGGGTGAGTGGGCAAAAAT
A
MDC012995.82 chr16:1352468..136
6194
249
(TA)
20
LG16
R- GACTCCGACTGCCGTGTTAT
LG16-1437845
F- GAATCGAACCCACATGCAG MDC018421.263 chr16:1437845..145
6844
232
(CT)
20
LG16
R- TTGTGTGCTCGAAAACATGC
LG16-LAR-SSR F- TGGATACAGGGAACCAAAA
CA
MDC015897.278 chr16:1526288..156
4541
238
(AT)
15
LG16
R- GCCCTTCCACTGCAAAATTA
LG16-1526288
F- TGTTGCTTTCTGTTTGGTTCA MDC015897.278 chr16:1526288..156
4541
168
(AG)
23
LG16
R- TTAATTGTGCTGCCTGCTTG
LG16-1568112
F- GGCATCCTAATTCCCTTGGT MDC002276.243 chr16:1568112..158
2774
241
(AT)
17
LG16
R- CAGGACCTGCAATTGTTACA
TC
LG16-1636246
F- CCCCAAAACCAAAACCCTA MDC001111.374 chr16:1636247..164
9334
231
(CT)
15
not
determined
R- GGACTCCTCGGTGTTGATGT
LG16-1661111
F- CGTCATTGTTGCAGGGTATG MDC006784.248 chr16:1661111..168
3666
153
(AG)
20
LG16
R- GCTTTCCTGCCACCACTACT
LG16-1728791
F- TTTGTCATGCTCGTTTCCTG MDC014193.228 chr16:1728791..176
0724
247
(AG)
13
LG16
R- GCTTAATTCTCGTGCCTTCG
LG16-1788357
F- GCACAGAGAGTCATGCCAGA MDC018602.176 chr16:1788357..182
9914
222
(AT)
22
LG16
R- TGACAACACATCAGCCAAAA
LG16-1860066
F- GCAGTCTATCATCCCAGGAC
A
MDC027868.28 chr16:1860066..187
2585
243
(GA)
14
LG16
R- CAACCACAGGTCCACAACAC
Note:
z
: Product size and SSR motif ampified from each primer pair were calculated from ‘Golden Delicious’ genome sequence