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Tree Genetics and Molecular Breeding 2014, Vol.4, No.1, 1
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vermifuge and the stem bark is useful in curing tapeworm
infection (Sambhandharaksa et al., 1962, Charoenlarp et
al., 1981).
A. lakoocha
extract is also reported to have
cosmetic ingredients (Tengamnuay et al., 2006).
With all these potential uses in pharmaceutical
industry, there is a need for commercial orcharding
and area expansion for which conventional methods of
sexual and vegetative propagation have proved to be
futile because of the inherent properties of poor seed
viability and low germination percentage and poor
response to vegetative means of cutting or grafting
techniques (Napier and Robbins, 1989). This prompts
for alternative approaches of developing propagules of
A. lakoocha
in large scale and micropropagation has
been a promising tool that has been reported to be
fruitful in developing successful plantlets.Where in
the conventional propagation methods had been less
successful or resulted in complete failure. There are
few reports on micropropagation of
A. lakoocha
using
in vitro
raised seedlings (Joshee et al., 2002; Rahman
and Amin, 1995) and some reports on jackfruits
(
Artocarpus heterophyllus
Lam.) by Abd El-Zaher
(2008), Khan et al. (2010), Ashrafuzzaman et al.
(2012). We report a rapid and efficient micropro-
pagation protocol using nodal explants from 8 years
old tree, for producing higher number of plantlets.
This prototype system will facilitate commercial
production of micropropagated plantletsof
A. lakoocha
as a source of providing genuine planting material.
1Result and Analysis
1.1 Shoot bud induction and multiplication
Explants cultured in MS+BAP 13.33+IAA 2.28+GA
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0.577 µM recorded early bud induction in about 7.33
days which showed longer axillary shoot (4.567 cm/
explant) and heighest number of leaves (6.667
leaves/explant) (Table 1) than other treatments.
However, MS+BAP 13.33+IAA 1.14 µM containing
multiplication medium produced 6.667 microshoots/
explant which also showed maximum number of
leaves (8.667 leaves/explant) and microshoot length
(3.967 cm/explant) (Table 2).
Table 1 Effect of different concentration of plant hormones on
in vitro
shoot bud induction
Treatments (µM)
Days taken for
bud induction
Average no. of
leaves/explant
Average length of
axillary shoots (cm)
Control (MS)
17.33
1.33
0.58
MS+BAP 4.44+IAA 1.14+GA
3
0.57
13.66
3.33
1.33
MS+BAP 4.44+IAA 2.28+GA
3
0.57
13.33
3.33
1.73
MS+BAP 8.88+IAA 1.14+GA
3
0.57
11.33
4.66
2.83
MS+BAP 8.88+IAA 2.28+GA
3
0.57
10.33
4.33
2.43
MS+BAP 13.33+IAA 1.14+GA
3
0.57
8.00
5.66
4.25
MS+BAP 13.33+IAA 2.28+GA
3
0.57
7.33
6.66
4.56
MS+BAP 17.76+IAA 1.14+GA
3
0.57
8.66
4.33
3.53
MS+BAP 17.76+IAA 2.28+GA
3
0.57
9.00
4.66
3.23
SE(m)±
0.44
0.38
0.36
CD (0.05)
1.33
1.15
1.09
Note: Values in these columns indicates average values of 3 replicates
Table 2 Effect ofdifferent concentration of cytokinin and auxin ratio on shoot multiplication
Treatments (µM)
No. of microshoots/
explant
No. of leaves/
explant
Length of microshoots/
explant (cm)
Control (MS)
1.333
2.333
0.583
MS+BAP 2.22+IAA 1.14+ADS 2.71
1.667
3.66
1.243
MS+BAP 2.22+IAA 2.28+ADS 2.71
2.667
4.000
1.733
MS+BAP 4.44+IAA 1.14+ADS 2.71
3.333
4.333
2.033
MS+BAP 4.44+IAA 2.28+ADS 2.71
4.333
4.667
2.633
MS+BAP 8.88+IAA 1.14+ADS 2.71
5.667
6.667
2.820
MS+BAP 8.88+IAA 2.28+ADS 2.71
5.333
7.333
2.567
MS+BAP 13.33+IAA 1.14+ADS 2.71
6.667
8.667
3.967
MS+BAP 13.33+IAA 2.28+ADS 2.71
5.667
8.000
3.367
SE(m)±
0.333
0.294
0.192
CD (0.05)
0.998
0.880
0.574
Note: Values in these columns indicates average values of 3 replicates