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Tree Genetics and Molecular Breeding 2014, Vol.4, No.1, 1
-
6
http://tgmb.sophiapublisher.com
1
Research Report Open Access
Micropropagation of
Artocarpus lakoocha
Roxb (Monkey jack) through Shoot
Bud Culture
Geetika Pant
1
, Umesh Kabade
2
1. Department of Biotechnology, Indian Academy Group of Institutions, Bangalore, India
2. Plant Tissue Culture, Arya Agribiotech and Research Centre, Santokpura, Anand, Gujarat, India
Corresponding authors email: way2geetika@gmail.com
Authors
Tree Genetics and Molecular Breeding, 2014, Vol.4, No.1 doi: 10.5376/tgmb.2014.04.0001
Received: 06 Mar., 2014
Accepted: 11 Mar., 2014
Published: 17 Mar., 2014
Copyright
©2014 Pant et al. This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Pant et al., 2014, Micropropagation of
Artocarpus lakoocha
Roxb (Monkey jack) through Shoot Bud Culture, Tree Genetics and Molecular Breeding, Vol.4,
No.1
-
6 (doi: 10.5376/tgmb.2014.04.0001)
Abstract
The Lakoocha (
Artocarpus lakoocha
Roxb
.
), popularly known as “monkey jack”, is called as ‘
barhal’
in India, belongs to
family Moraceae and comprises of many species of woody trees. The present study was aimed to develop a micropropagation
protocol for Lakoocha towards commercial mass production of planting material which permits rapid and easy multiplication rates,
outwits genotype barriers, offers opportunities for continuous availability of genuine planting material with economic viability. We
report here a prototype system of micropropagated plantlets of Lakoocha through shoot bud culture within 6 months catering to those
parameters of commercial mass multiplication. Nodal explant with an axillary bud used as explant; showed
in vitro
bud induction
within 7.33days in MS+BAP 13.33+IAA 2.28+GA
3
0.57 µM. The maximum shoot multiplication (6.66 shoots/explant) was observed
in MS medium supplemented with BAP 13.33 +IAA 1.14 µM. The microshoots were maintained in MS basal medium (BAP 0.89
µM) supplemented with Casein Hydrolysate 100 mg/l and then subsequently rooted in ½ MS medium supplemented with IBA
9.85+BAP0.89 µM+Activated charcoal 500 mg/l which resulted in 88.16% rooting. The
in vitro
rooted plants were primary hardened
on autoclaved Sand : Soil : Peat moss (1:1:2) drenched with ½ strength MS nutrients in plastic cups covered with transparent
polyethylene bags to prevent excess water loss for 3 weeks in growth room at 25±2
0
C and then transferred to shade net house (50%
shade 70%±5% RH) for secondary hardening. All plants showed 88.33% survival during acclimatization. The plants that were field
established showed > 85% survivability, which is under further evaluation for fruiting and quality characters.
Keywords
Prototype; Micropropagation; Artocarpus lakoocha; Monkey Jack
Abbreviations:
IAA- Indole-3-Acetic Acid,
IBA- Indole-3-Butyric Acid, BAP- Benzyl Amino Purine, GA
3
-Gibbrellic acid, ADS-
Adenine sulphate, ½ MS (Half strength MS), MS (Full strength MS)
Introduction
The lakoocha (
Artocarpus lakoocha
Roxb.), most
popularly known as “
monkey jack
” or ‘
barhal
’ and
pachoo phanas
in local language (bangalore) in India,
belonging to family moraceae, originated from South
East Asia and is widely distributed in the humid
sub-himalayan regions of India. Itis regarded as
underutilized fruit, although “lakoocha” is grown
mainly in Uttar Pradesh, Bengal, Khasi Hills and
Western Ghats, because of lack of commercial
orchards, lesser consumer preference and poor
marketability. Recent commercial interest in several
tropical and subtropical underutilized fruits has
resulted in an increased cultivation area in Asia and
other regions of the developing world due to high
level of vitamins and minerals has been recorded in a
number of underutilized fruits. The export of fruits
from Asia alone has been increasing by a little over
10% annually (Singh, 1993). Most commonly, leaves
of these trees serves as green fodder for cattle due to
their rich source of crude protein, fibers and mineral
(Joshee et al.,
2002). These trees grow upto 6 to 12 m
tall with large, leathery and deciduous leaves but with
fruits of no edible value. Yet the choice of the fruit in
Ayurveda and Siddha medicines are due to their
multivarious active principles. The medicinal
principles that have been well documented in
A.
lakoocha
includes tannins (Doss et al., 2009),
flavonoids (Mandalari et al., 2007; Maneechai et al.,
2012), saponins (Avato et al., 2006), terpenoids
(Funatogawa et al., 2004) and alkaloids (Navarro et al.,
1999). Fruit pulp is used as liver tonic and bark extract
is used in external ailments like heals boils, cracked
skin and pimples (Shailendraet al., 2010). The stem is