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Impact of Salt Stress (Nacl) on Seed Germination, Photosynthetic Pigments of Green Gram Cultivars of Co6 and Co8
42
1.5 Vigour index
The vigour index of the seedlings was calculated using
the following formula proposed by Abdul-Baki and
Anderson (1973) and expressed as per cent.
Vigour Index=(Shoot length+Root length)× Germi-
nation percentage
1.6 Photosynthetic pigments
The chlorophyll content was estimated following the
method suggested by Yoshida et al. (1971) and
expressed as mg g
-1
fresh weight.
1.7 Procedure
Take 250 mg of leaf sample is macerated with 10ml of
80% acetone using a pestle and mortar and the extract
is centrifuged at 3000 rpm for 10 minutes. The
supernatant solution is transferred into a 25ml
volumetric flask and made up to 25ml using 80%
acetone. Then the color intensity of the green pigment
is read at 480nm, 510nm, 645nm, 663nm and 652nm
for chlorophyll a, chlorophyll b and total chlorophyll
content respectively using Spectrophotometer.
1.8 Calculation
Total Chlorophyll=Chlorophyll a+Chlorophyll b
2 Result and Discussion
2.1 Germination percentage
The result on seed germination percentage was
significantly differed in all the treatments. Among the
treatments, T
1
showed highest germination percentage
in green gram both CO
6
and CO8 (99.7 and 99.7),
which was followed by T
2
, T
3
, T
4
and T
5
. The lowest
germination percentage was recorded in T
9
, T
10
and T
11
treatments. The treatments of T
9
and T
11
in CO
6
and T
2
and T
3
, T
7
and T
8
were on par with each other (Table 3).
Table 3 Effect of salt stress (NaCl) on germination percentage, shoot length, root length and vigour index of green gram
Treatments
Germination (%)
Shoot length (cm)
Root length (cm)
Vigour index
CO 6
CO8
CO 6
CO8
CO 6
CO8
CO 6
CO8
T
1
99.7
99.7
8.74
10.00
5.92
5.90
1460.85
1584.43
T
2
89.0
89.0
9.60
10.32
5.56
4.84
1349.00
1349.00
T
3
89.0
84.0
7.74
9.26
5.48
5.64
1176.34
1251.38
T
4
94.0
79.0
8.64
9.30
5.04
6.24
1285.67
1227.45
T
5
79.0
79.0
4.76
8.14
5.04
5.50
773.99
1077.35
T
6
84.0
79.0
4.02
5.54
4.80
5.52
740.66
873.53
T
7
74.0
79.0
6.64
6.22
3.94
4.42
782.72
840.35
T
8
74.0
84.0
4.14
3.16
4.36
3.82
628.80
586.10
T
9
64.0
84.0
4.38
4.24
3.82
5.44
524.63
812.90
T
10
69.0
79.0
2.16
3.56
3.80
3.64
411.06
568.59
T
11
64.0
54.0
1.72
3.96
3.52
4.04
335.19
431.86
Mean
80.0
80.9
5.7
6.7
4.4
5.0
1460.85
1584.43
SE.d
0.791
0.791
0.419
0.419
0.419
0.419
0.160
0.163
CD (0.05)
1.641
1.641
0.870
0.870
0.870
0.870
0.302
0.303
The increased seed germination percentage was
noticed in control (distilled water) treated seeds and
very less reduction was observed in T
2
to T
5
treatments in the range of 11.2 percent over the T
11
.
The highest reduction percent of 35% were observed
in T
10
, T
11
, and T
12
treated seeds. Enzyme called α –
amylase which is essential for seed germination is
inhibited due to salt stress. Starch to sugar conversion
occurs during germination is also affected by salinity.
It also delays the synthesis of nucleic acids and
RNAase (Tester and Davenport, 2003).
2.2 Shoot length (cm) and Root length (cm)
The result on shoot length was significantly differed in
all the treatments. Among the treatments, T
2
showed
the highest shoot length in green gram both CO
6
and
W 1000
V
645) OD
(2.69
663)
at O.D
(14.7 a l
Chlorophyl
× -
× =
W 1000
V
663) OD
(4.68
645)
at O.D
(22.9 = b l
Chlorophy
× -
×
PLANT GENE AND TRAIT