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for survival, the loss of biodiversity escalates. Salinity
also reduces the productivity of crops and the
sustainability of agriculture. Based on the above
constraints, we are taken the following objectives,
Screening of Green gram varieties for NaCl stress
tolerance through morphological analysis.
To determine the physiological basis of NaCl stress
tolerance in Green gram varieties.
1 Materials and Methods
The experiment was carried out at Vanavarayar
Institute of Agriculture (TNAU affiliated), Pollachi,
Tamil Nadu, India during 2013-2014. The experiment
consists of ten treatments with three replications were
laid out in completely randomized block design with
two cultivars of CO5 and CO6. Seeds of green gram
varieties obtained from Department of Pulses, Tamil
Nadu Agricultural University, Coimbatore, were used
for the study and the details of the varietal characters
were listed in Table 1. Green gram varieties (Table 1)
were screened for tolerance to various levels of
sodicity stress, based on germination per cent,
seedling growth and vigour index, seeds were allowed
to germinate in Petri dishes. The germination medium
was prepared following the procedure mentioned
below. Petri dishes were sterilized using 0.01% HgCl
2
and 70% ethanol and finally washed with distilled
water. Before placing the germination sheet, Petri
dishes were cleaned thoroughly with a cotton swab.
The surface sterilized (70% ethanol) 15 seeds from
each variety were placed in each Petri dish. For
imposing sodicity (11 levels as considered as
Treatments) stresses, sodium chloride (NaCl) solution
at the concentration of T
1
: control (without NaCl),
T
2
:10, T
3
:20, T
4
:30, T
5
:40, T
6
:50, T
7
:60, T
8
:70, T
9
:80,
T
10
:90 and T
11
:100 ppm were prepared. The seeds
were allowed to germinate, by sprinkling the salt
solution of 10 ml each in alternate days. Distilled
water was used for maintaining the control. The pH
and EC details of the salt solution used for experiment
were given in Table 2.
Table 1 Varietal details
S. No
Crop
Variety
Duration (days)
Yield (kg/ha)
Rainfed
Irrigated
1.
Green gram
CO6
62~67 days
900
1050
2.
CO8
70 days
900
1050
Table 2 pH and EC of the salt solution used for experiment
S.No
(NaCl) Salt solution (ppm)
pH
EC (dS/m)
1
0
7.1
0.42
2
10
7.3
2.0
3
20
7.5
4.1
4
30
7.6
5.3
5
40
7.7
6.9
6
50
7.8
7.0
7
60
8.2
7.6
8
70
8.4
8.1
9
80
8.7
8.5
10
90
9.2
9.00
11
100
9.5
10.00
1.1 Observation recorded:
The germination percentage, root length, shoot length
and Vigour index were measured at 15
th
DAS and
chlorophyll ‘a’ ‘b’ and total chlorophyll content was
estimated based on the procedure given by Yoshida et
al. (1979) and expressed as mg/g fresh weight.
1.2 Germination percentage
The germinability was recorded on the fifteenth day
after sowing (DAS) and number of seeds germinated
was expressed as per cent.
1.3 Seedling shoot length
On 15
th
DAS, seedlings from each replication were
carefully removed at random. Length of shoot was
measured from the collar region to the tip of the
longest leaf and expressed as cm.
1.4 Seedling root length
Root length of the seedlings was measured from the
base of the stem to the tip of the longest root and
expressed as cm.
100
n
germinatio
for
kept
seeds of
Number
seeds
germinated
of
Number
=
percentage
n
Germinatio
PLANT GENE AND TRAIT