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Medicinal Plant Research 2014, Vol.4, No.3, 30
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34
http://mpr.sophiapublisher.com
30
Research Report Open Access
Antioxidant and Cytotoxic Activity of
Physalis peruviana
Tuğçe DEMİR
1
, Mehmet Özgün ÖZEN
1,2
, E. Esin HAMEŞ-KOCABAŞ
1
1
.
Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey
2
.
Department of Bioengineering, Faculty of Engineering, Gümüşhane University 29100 Gümüşhane, Turkey
Corresponding author email: esin.kocabas@ege.edu.tr; esinkocabas@gmail.com;
Authors
Medicinal Plant Research, 2014, Vol.4, No.4 doi: 10.5376/mpr.2014.04.0004
Received: 17 Feb., 2014
Accepted: 25 Feb., 2014
Published: 28 Feb., 2014
Copyright
© 2014 DEMİR et al. This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
DEMİR et al., 2014, Antioxidant and Cytotoxic Activity of
Physalis peruviana
n, Vol.4, No.4 30-34 (doi: 10.5376/mpr.2014.04.0004)
Abstract
Physalis peruviana
has widely used in folk medicine as a medicinal herb for treating diseases since ancient times. It is a
pharmacologically valuable natural product due to the presence of biologically active compounds such as phytosterols, vitamins,
essential minerals, withanolides and physalins. In this study, we aimed to observe the antioxidant capacity and cytotoxic activity of
edible parts of
P. peruviana
on different cancer cell lines [human colon adenocarcinoma cell line (HT-29), human prostate
adenocarcinoma cell line (LNCap), human hepatoma cell line (Hep3B), human breast adenocarcinoma cell line (MCF-7), human
neuroblastoma cell line (SH-SY5Y), human osteosarcoma cell line (SaOS-2)] and a non-cancerous kidney epithelial cells from African
green monkey (Vero) cell line. Antioxidant activity of the fruit extracts was determined with DPPH (1,1-diphenyl-2-picrylhidrazil)
radical scavenging method and cytotoxic effect was observed by using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium
bromide) assay. For antioxidant capacity of the crude extract, IC
50
value was calculated as 0.43±0.003 mg/ml. IC
50
values of
cytotoxicity for HT-29, Hep3B, SaOS-2 and SH-SY5Ycell lines for 48 h were determined as 40.79, 24.92, 15.44 and 44.24 µg/ml,
respectively. Fruit extract of
P. peruviana
had no cytotoxic effect on MCF-7, LNCap and Vero cell lines.
Keywords
Antioxidant activit; Cytotoxic activity; DPPH;
Physalis peruviana
Background
Physalis peruviana
is a member of the family
“Solanacaceae” and genus “
Physalis
”. Homeland of
Physalis peruviana
is Andes region and nowadays
South Africa and Colombia have the biggest market
share as producers’ worldwide.
P. peruviana
is known
with different names such as uchuva (in Columbia),
uvilla (in Ecuador), aguaymanto (in Peru), topotopo
(in Venezuela) and in English speaking countries it is
known as “goldenberry” and “cape gooseberry”
(Salazar et al., 2008; Puente et al., 2011). In Turkey,
this fruit have become very popular and is called as
“altın çilek”. P. peruviana has widely used in folk
medicine as a medicinal herb for treating diseases
such as malaria, asthma, hepatitis, dermatitis, diuretic,
rheumatism and cancer (Wu et al., 2004; Ramadan,
2011).
Recently,
P. peruviana
has become attractive because
of its nutritional and medicinal properties. According
to the published reports, presence of biologically
active compounds such as phytosterols, vitamins,
essential minerals, withanolides and physalins make it
an important food supplement with medicinal
properties (Puente et al., 2011). Phytosterols are
known for especially their antioxidant capacity and
reports show fruits of
P. peruviana
have antioxidant
capacity (Wu et al., 2006; Vasco et al., 2008; Puente et
al., 2011). The reason of orange colour of fruit is
β-carotene which is the main active component of
vitamin A. β-carotene and vitamin C content of
P.
peruviana
provides its anticancerous function,
associated with preventing accumulation of free
radicals in tissues. Especially, withanolides and
physalins are very important biologically active
components for anti-inflammatory, antimicrobial, anti
tumor, immunomodulatory and antiparasitic properties.
Cytotoxic and antiproliferative effects of
P. peruviana
leaves, stems and/or whole plant on different cell lines
such as colon cancer, chronic myeloid leukomia, lung,
breast and liver cancer cells were also reported (Wu et
al., 2004; Zavala et al., 2006; Lan et al., 2009; Wu et
al., 2009).
In this study, we aimed to observe the antioxidant and
cytotoxic activity of edible parts of
P. peruviana.