分子植物育种
(
网络版
), 2016
年
,
第
14
卷
,
第
1016
-
1023
页
Fenzi Zhiwu Yuzhong (Online), 2016, Vol.14, 1016
-
1023
Copyright © 2016 BioPublisher 1016
研究报告
Research Report
烤烟易烤性连锁标记的适用性验证
王春凯
1,2
,
任民
1
,
徐秀红
1,3
,
许家来
3
,
王传义
1
,
李青山
1,2
1.
中国农业科学院烟草研究所
,
农业部烟草生物学与加工重点实验室
,
青岛
, 266101
2.
中国农业科学院研究生院
,
北京
, 10081
3.
山东烟草研究院
,
济南
, 250098
通讯作者
作者
分子植物育种
, 2016
年
,
第
14
卷
,
第
3
篇
doi: 10.5376/mpb.cn.2016.14.0003
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用,版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
:
王春凯等
, 2016,
烤烟易烤性连锁标记的适用性验证
,
分子植物育种
(online), 14(3): 1016-1023 (doi: 10.5376/mpb.cn. 2016.14.0003)
引用格式
(
英文
)
:
Wang et al., 2016, Validation on Applicability of Markers Linked to Easy Curing of Cured Tobacco, Fenzi Zhiwu Yuzhong (online) (Molecular Plant
Breeding), 14(3): 1016-1023 (doi: 10.5376/mpb.cn2016.14.0003)
摘
要
烤烟的易烤性影响了烘烤过程中的变黄过程,直接影响烤后烟叶的外观质量。本研究对
5
个与烤烟易烤性紧密连
锁的分子标记进行了有效性检测。结果表明,在云烟
85
和大白筋
599
组合的
F2
群体中利用亲本带型进行分离群体分组,
除标记
PT53143
外,其余
4
个标记其组间易烤性差异均达到显著以上水平;在自然群体中,以易烤品种云烟
85
的等位基因
型为优异基因型,根据供试品种含有优异基因型位点的数量多寡可将
24
份品种分为两组,且两组之间易烤性差异达到了显
著水平,进一步的遗传多样性分析揭示了易烤性较好一组的供试位点明显趋向保守,存在典型的选择牵连效应,推测供试位
点附近极有可能存在与易烤性调控有关的基因。本研究不但验证了供试标记的可用性,同时也提出了一种利用供试标记进行
烤烟易烤性分子鉴定和筛选的策略,为今后烤烟品种易烤性改良和新品种选育提供了一定的技术基础。
关键词
烤烟
;
易烤性
; SSR
分子标记
;
适用性
Validation on Applicability of Markers Linked to Easy Curing of Cured
Tobacco
Wang Chunkai
1,2
Ren Min
1
Xu Xiuhong
1,3
Xu Jialai
3
Wang Chuanyi
1
Li Qingshan
1,2
1. Tobacco Research Institute of CAAS, Key laboratory of Tobacco Biology and Processing of Ministry of Agriculture, Qingdao, 266101;
2. Graduate School of CAAS, Beijing, 100081;
3. Tobacco Research Institute of Shandon, Jinan, 250098
Corresponding author
Authors
Abstract
Exterior quality of cured tobacco was directly subject to easy curing that affects the yellowing process of curing. Five
markers linked to easy curing were tested in efficiency. The results showed that the individuals in F2 population by crossing
Yunyan85 and Dabaijin599 were grouped according to the banding patterns of the two parents. Difference of easy curing was showed
in significant level or above between two groups in four markers except for the marker PT53143. In natural population where the
allele of easy curing cultivar Yun85 was defined as excellent genotype, 24 materials were grouped into two according to the number
of excellent allele that the tested cultivars have, and difference between the two groups was in significant level. Furthermore, the
genetic diversity analysis revealed obviously that the tested locus of the better group leaned into conservative, which was a typical
symbol of hitchhiking effects. It was speculated that genes related to regulating easy curing exists probably near these locus. In the
research, the availability of the tested markers have been verified and strategies of using tested markers to make identification and
selection have been suggested to supply a technique foundation for promotion of easy curing potential and breeding.
Keywords
Cured tobacco; Easy curing; SSR molecular marker; Applicability
研究背景
烟草作为重要的经济作物,其烟叶品质备受
关注,而烘烤过程是影响烟叶品质的关键环节。
生产实践中,根据烟叶的烘烤特性来制定相应的
烘烤工艺。易烤性作为烘烤特性的重要组成部分,
体现在烘烤过程中的变黄期中,烟叶的变黄、脱
水的难易程度及同步程度
(
倪超等
, 2011)
,主要受
收稿日期:
2016
年
01
月
13
日
接受日期:
2016
年
02
月
15
日
发表日期:
2016
年
02
月
20
日
基金项目:本研究由国家烟草专卖局烟草基因组计划重大
专项
(110201301008[JY-08])
;中国烟草总公司科技重点项目
(110201002017)
和国家公益 性行业
(
农业
)
科研 专项
(201203091)
共同资助。