分子植物育种
(
网络版
), 2016
年
,
第
14
卷
,
第
1001
-
1006
页
Fenzi Zhiwu Yuzhong (Online), 2016, Vol.14, 1001
-
1006
Copyright © 2016 5
th
Publisher 1003
表
1
农杆菌介导的高粱遗传转化
Table 1 Details of
Agrobacterium
-mediated genetic transformation in sorghum
基因型
Genotype
外植体
Explant
农杆菌株
Strains of
Agrobacterium
选择标记基因
Selective marker
gene
报告基因
Reporter
gene
参考文献
Reference
PHI391,
P898012
幼胚
Immature embryos
LBA4404
bar
gus
Zhao et al
.
, 2000
Tx 430, C401,
CO25,
Wheatland
幼胚
Immature embryos
EHA101, EHA105,
AGL1
-
Gus
,
gfp
Jeoung et al., 2002
P898012
幼胚
Immature embryos
LBA4404
hpt
gus
Carvalho et al.,2004
Tx430, C401
幼胚
Immature embryos
EHA101
-
gfp
Gao et al., 2005a
Pioneer 8505,
C401
幼胚
Immature embryos
EHA102
manA
gfp
Gao et al., 2005b
Tx430, C2-97
幼胚
Immature embryos
NTL4
npt II
gus
Howe et al., 2006
Sensako
85/1191
幼胚
Immature embryos
LBA4404
hpt
gus
Nguyen et al
.
, 2007
296 B
茎尖
,
叶
Shoot apices,
Leaves
LBA4404, EHA105
-
gus
Verma et al., 2008
C401, 296 B,
Tx430,
P898012
幼胚
Immature embryos
LBA4404, EHA101
manA
gfp
Gurel et al., 2009
P898012
幼胚
Immature embryos
EHA101
bar
gus
Lu et al., 2009
115, ICS21B,
5-27
幼穗
Immature
inflorescence
EHA105
Hpt
,
npt II
gus
Zhang et al., 2009
TNS586, CO25
幼胚
Immature embryos
LBA4404, EHA105
Bar
,
hpt
gus
Indra Arulselvi et
al
.
, 2010
M 35-1
成熟胚
,
幼苗
,
叶
,
幼穗
Mature embryos,
Young seedling,
Leaves, Immature
inflorescence
LBA4404
hpt
gfp
Shridhar
et al
.
, 2010
BABUSH,
MN-3025
幼穗
BABUSH,
MN-3025
P898012
幼胚
Immature embryos
EHA101, LBA4404
manA
gfp
Gurel et al., 2012
APK 1
茎尖
Shoot apices
LBA4404
hpt
gus
Ignacimuthu et al.,
2014
TX430
幼胚
Immature embryos
LBA4404, AGL1
bar, manA
DsRed
，
yfp
Wu et al., 2014
注
: bar:
双丙氨膦抗性
; gfp:
绿色荧光蛋白
; gus: β-
葡萄糖苷酸酶
; hpt:
潮霉素磷酸转移霉
; npt II:
新霉素磷酸转移酶
; manA:
磷酸甘露糖异构酶
; DsRed:
红色荧光蛋白
; yfp:
黄色荧光蛋白
Note: bar: phosphinothricin acetyl transferase; gfp: green fluorescence protein; gus: β-glucuronidase, hpt: hygromycin
phosphotransferase; nptII: neomycin phosphotransferase; manA: phosphomannose isomerase; DsRed: red fluorescence protein; yfp:
yellow fluorescence protein
