Basic HTML Version
Maize Genomics and Genetics 2012, Vol.3, No.1, 1
-
5
ISSN 1925-1971 http://mgg.sophiapublisher.com
5
Figure 7 The construction of pHorD-
GBSS
Ⅰ
expression vector
gene of plant expression vector, specific premiers were
designed. Primer sequences are as follows: Test-F:
5'
-
AGAACAGACCAAGATACAAACG
-
3'; Test-R:
5'
-
ATAGGGACGAGGCGAAGA
-
3'. Used the geno-
mic DNA extracted from leaves of regenerated plant as
template to detect the
bar
gene in the regenerated
plants. PCR amplification was carried out with the
specific primers, which were designed based the
fragments of
GBSS
Ⅰ
gene and promoter
HorD
. Took
the recombinant plasmid pHorD-
GBSS
Ⅰ
as the
positive control, the untransformed plants as negative
control, and H
2
O as blank control, we detected by
PCR method.
Author contributions
Rongxi Zhou and Ying Wu are the executors of experimental
design and experimental study, Hongda Zou is the guidance of
the vectors design, Hongkui Liu assisted transplant seedlings
and provided technical post-management, Shipeng Li and
Xiaohui Shan guided and analyzed the single-post test data and
wrote the first draft paper; Shengzhong Su involved in the
experimental design; Yaping Yuan is in charge of the project
and experimental idea who participated in experimental design
and wrote and modified the paper. All authors have read and
agreed the final text of this article.
Acknowledgements
The research was funded by the National GMO Cultivation of
New Varieties of Major Projects fund (2009ZX08003
-
024B).
References
Chai X.J., Wang P.W., Guan S.Y., and Xu Y.W., 2005, Reducing the
amylopectin content through RNA interference manipulation, Zhiwu
Shengli Yu Fenzi Shengwuxue Xuebao (Journal of Plant Physiology
and Molecular Biology), 31(6): 625-630 http://dx.doi.org/10.1007/
s001220051191
Cho M.J., Choi H.W., Buchanan B.B., and Lemaux P.G., 1999, Inheritance
of tissue-specific expression of barley hordein promoter-
uid
A fusions
in transgenic barley plants, Theor. Appl. Genet., 98(8): 1253-1262
Kuipers G.J., Vreem J.T.M., Meyer H., Jacobsen E., Feenstra W.J., and
Visser R.G.F., 1992, Field evaluation of antisense RNA mediated
inhibition of GBSS gene expression in potato, Euphytica
,
59(1): 83-91
Li Y., 2011, Study on genetic transformation of
Isa
-H1 gene in Maize,
Thesis for M.S., Plant Sciences Institute, Jilin University, Supervisor:
Yuan Y.P., pp.57-70
Liu J.B., 2011, Study on transformation of AGPase gene in Maize, Thesis
for M.S., Plant Sciences Institute, Jilin University, Supervisor: Yuan
Y.P., pp.57-70
Oltmanns H., Frame B., Lee L.Y., Johnson S., Li B., Wang K., and Gelvin
S.B., 2010, Generation of backbone-free, low transgene copy plants by
launching T-DNA from the
Agrobacterium chromosome
, Plant Physiol.,
152(3): 1158-1166 http://dx.doi.org/10.1104/pp.109.148585
Pistón F., Shewry P.R., and Barro F., 2007, D hordeins of
Hordeum chilense
:
a novel source of variation for improvement of wheat, Theor. Appl.
Genet., 115(1): 77-86 http://dx.doi.org/10.1007/s00122-007-0542-0
Villand P., Anlen R., Olsen O.A., Lüthi E., Lönneborg A., and Kleczkowski
L.A., 1992, Pcr amplification and sequence of cDNA clones for the
small and large subunits of
ADPglucose
pyrophosphorylase
from
barley tissues, Plant Mol. Bio., 19(3): 381-389 http://dx.doi.org/
10.1007/ BF00023385
Visser R.G., Somhorst I., Kuipers G.J., Ruys N.J., Feenstra W.J., and
Jacobsen E., 1991, Inhibition of the expression of the gene for
granule-bound starch synthase in potato by antisense constructs, Mol.
Gen. Genet., 225(2): 289-296 http://dx.doi.org/10.1007/BF00269861
Wang Z.H., Kang W.M., and Zhang X., 2002, High starch corn and its
process utilization, Yumi Kexue (Journal of Maize Sciences), 10(3):
90-92
Xia H., Yandeau-Nelson M., Thompson D.B., and Guiltinan M.J., 2011,
Deficiency of maize starch-branching enzyme I results in altered
starch fine structure, decreased digestibility and reduced coleoptile
growth during germination, BMC Plant Biol., 11: 95 http://dx.doi.org/
10.1186/1471-2229-11-95
Zhang G.T., Lu D.C.C., Sun C.X., Liang R.Q., Yang F.P., and Zhang X.D.,
2010, Increasing of amylose content by sense RNA interference,
Huabei Nongxuebao (Acta Agriculturae Boreali-Sinica), 25(4): 92-96
Zhang Y., Xu X.H., Zhu Y.Q., Han P., and Li S.Q., 2005, Breeding
surveying and development prospect of high amylose, Yumi Kexue
(Journal of Maize Sciences), 13(1): 52-54