Legume Genomics and Genetics 2015, Vol.6, No.3, 1-7
6
4.2 Breeding Method
The beeding method followed was hybridization
followed by selfing and selection by pedigree method.
4.3 Nutritional and Antinutritional Studies
4.3.1 Nutritional studies
Includes, dry matter content, moisture content, and
crude protein which were estimated as per standard
methods (AOAC, 1990). While, estimation of crude
fiber was done by Sadasivam and Manickam (1992)
method, the total free amino acids was by Moore and
Stein (1948). methionine by Horn and Jones (1948),
tryptophan by Mertz et al. (1975) and the estimation
of
p
roline was done as per the method suggested by
Bates et al. (1973).
4.3.2 Determination of anti-nutritional Factors
The total phenolic content was estimated by slight
modification of the method of Bray and Thorpe (1954),
Trypsin inhibitor activity using BAPA N-benzyol- DL-
arginiine-P-nitroaniliide hydrochloride and tryspsin III
from bovine pancreas by Kakade et al. (1969) and the
estimation of Tannins was done as per the method
suggested by Sadasivam and Manickam (1992).
Acknowlement
Prof. (Mrs.) Shastri, Laxmi Naryan Institute of Technology,
Nagpur for her help in analyzing nutritional and antinutritional
factors in Lablab bean cultivars.
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