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Molecular Soil Biology (online), 2011, Vol.3 No.1
ISSN1925-2005
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JB1~JB22 in turn. 21 algal species have been purified
except JB12; 8 algae species were isolated from
garden nursery soil, recorded as CK1~CK8 in turn.
Most of the 30 algae species are single cell type, but
there are also filaments type (JB9), group type (such
as JB22); Cell morphology varied a lot, for example,
globe (such as CK5), ellipse (eg., CK1), ovate (such
as CK3), cylindrical (JB9), drum-type (JB10), spindle
(JB5), crescent (CK2); Some algae species had
significant flagella (eg., JB2, JB4, JB7, CK8); Most of
them are green, but some are yellow-green (JB9 and
JB10), brown (JB13, JB18), blue-green (JB15).
Morphological identification of algae is a long-term
and pain staking work, therefore, the algae species
were just indicated by numbers in this study.
1.2 Resistance analysis of algae
In order to study the resistance of algal, 29 algae
species (except JB12) purified were cultured in BBM
solid medium with different concentrations of NaCl
and NaHCO
3
.
In the blank medium and the BBM medium with
50 mmol/L NaCl stress, all of the algae could grow;
When the concentration of NaCl was 550 mmol/L,
algae species were subject to suppression and could
not grow except CK1, CK2, JB6, JB9, JB14, JB16,
JB17, JB19; When in 1 mol/L NaCl, only JB6 and
JB19 could survival (Figure 1).
In 100 mmol/L NaHCO
3
, the control algae species
were suppressed seriously except CK2, while those of
alkali spot soil tended to raise except JB2, JB5, JB11,
JB21, JB22; But in 200 mmol/L NaHCO
3
, CK2 was
inhibited, while the others changed little; Under 400
mmol/L NaHCO
3
stress, CK1 and CK2 turned yellow,
show- ing mortality trends, JB6, JB19 showed
strongest resistance, followed by JB13, JB14, JB16
and JB17 (Figure 2).
In conclusion, JB19 and JB6 showed strongest
resistance to both NaCl stress and NaHCO
3
stress,
which could tolerate 1 mol/L NaCl stress and more
than 400 mmol/L NaHCO
3
stress, while the control algae
species CK1 and CK2 could only tolerate 550 mmol/L
NaCl stress and 200 mmol/L NaHCO
3
stress.
Figure 1 Analysis of algae expressed in different NaCl stresses
Note: Algae species included JB1~JB22 (except JB12) and
CK1~CK8, incubated as described in Materials and Methods;
Serial dilutions were spotted onto solid BBM medium
supplemented without or with additional NaCl (50 mmol/L,
550 mmol/L and 1 mol/L); Growth was observed one week later
2 Conclusions
(1) We isolated 30 algae species eventually, 22 of
which were isolated from alkali spot soil in northeast
China, and 8 algae species from garden nursery soil.
29 algae species had been purified.
(2) After resistance screening of 29 algae species by
NaCl stress and NaHCO
3
stress, we found their
growth conditions were different from each other at
different stress or different stress concentration
gradient. Algae species of alkali spot soil showed
stronger resistance than those of nursery soil.
(3) Some algae were strongly inhibited by lower concentra-
tion of sodium bicarbonate, but some were promoted.