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Molecular Soil Biology (online), 2011, Vol.3 No.1
ISSN1925-2005
http://msb.sophiapublisher.com
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Research Letter Open Access
Isolation and Resistance Analysis of Algae of Alkali Spot Soil
Juan Wang
1
, Wei Shi
1
, Takano Tetsuo
2
, Shenkui Liu
1,2
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education,; Alkali Soil Natural Environmental Science
Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, 150040;
2. Asian Natural Environmental Science Center (ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Corresponding author email:
shenkuiliu@nefu.edu.cn;
Author
Molecular Soil Biology, 2011, Vol.3 No.1 doi: 10.5376/msb.2011.03.0001
Received: 08, Jun., 2011
Accepted: 21, Jun., 2011
Published: 28, Jun., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wang et al., 2011, Isolation and Resistance Analysis of Algae of Alkali Spot Soil, Molecular Soil Biology, doi:10.5376/msb.2011.03.0001
Abstract
Algae can survive in a very wide range environment and have high adaptability for a variety of adversity. In present study,
we selected alkali spot soil in Anda City, Heilongjiang Province as the algae research plots. In addition, we selected garden nursery
soil in Northeast Forestry University as the control. With streak plating method, we isolated 30 algae species eventually, 29 of which
had been purified. After resistance screening of 29 algae species by NaCl stress and NaHCO
3
stress, we found their growth
conditions were different from each other at different stress or different stress concentration gradient. Algae species of alkali spot soil
showed stronger resistance than those of nursery soil. Moreover, some algae were strongly inhibited by lower concentration of
sodium bicarbonate, but some were promoted.
Keywords
Alkali spot soil; Soil algae; Salt-stress; Isolation and identification; Resistance
Background
Algae can survive in a very wide range environment,
they can be found whether in soils, rocks and caves, or
in permanent snow and ice fields, even on living
animals and plants, algae are almost everywhere
(Hoffmann, 1989). Soil habitats are the most important
non-aqueous ecosystems for algae (Zenova et al.,
1995). Soil algae include the algae living in
water-terrestrial, true-terrestrial, native, hidden and
algae on rock (Metting, 1981).
Since 1990s, more and more scientists are doing
research on the ecology and application of soil algae;
(Mazor et al., 1996); Current research focus on the use
of soil algae as bio-fertilizer, pesticides testing
organism and the research on soil improvement and
desertification control (Whitton and Potts, 2000)
；
In
addition, in order to explore the possibility of life
beyond the earth and the origin of life, polar ecology
and the resistance mechanisms of pioneer algae
species are conspicuous, and have made outstanding
achievements (Scherer et al., 1988; Davey and Rothery,
1993; Young and Fuank, 1996; Gorton et al., 2001;
Holzinger and Lütz, 2006; Remias et al., 2010;
Tanabe et al., 2011).
Although there are many studies on soil algae
(Johansen, 1993; Sukala and Davis, 1994; Tsujimura
et al., 2000; Lukešová, 2001; Neustupa, 2001; Zancan,
2006; El-Gamal et al., 2008), the studies on the diversity
and resistance of algae of alkali spot soil in northeast
China are few. The salinity volume of the saline soil
in Anda City, Heilongjiang Province, is 0.3% to 1.5%,
and the soil pH is around 10, and the dominate soil
soluble salt are bicarbonate and carbonate. Because of
the high soluble salt content, general biology can
hardly survive. However, some algae are able to grow
and multiply in such environment. Algae have
potential resistance-related genetic resources, the aim
of this study on the isolation and resistance-
identification of the algae of alkali spot soil was to
filter out the algae of high resistance, which is the
foundation of the screening of the salinity-related
genes.
1 Results and Analysis
1.1 The algae species from identification and
purification
We isolated 30 algae species eventually, 22 of which
were isolated from alkali spot soil, recorded as