Basic HTML Version
Molecular Soil Biology (online), 2011, Vol. 2 No.2, 9
-
14
ISSN1925-2005
http://msb.sophiapublisher.com
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Figure 2Alignment of deduced amino acid sequences of
PutSTE24
with its homology in
Arabidopsis thaliana
,
Zea mays
and
Ricinus Communis
Note: The upper box indicates an HEXXH Zn
2+
-metalloprotease signature; The lower box indicates a KKXX ER membrane retention
signal; Accession number of
Arabidopsis thaliana
: At4g01320,
Zea mays
: 100286144 and
Ricinus Communis
: 8286673
Figure 3 Growth assay of yeast expressing
PutSTE24
and
AtSTE24
in the stress of AlCl
3
Note: Yeast cells containing pAUR123, pAUR123
-
PutSTE24
and pAUR123
-
AtSTE24 were, respectively, incubated as
described in materials and methods; Serial dilutions were
spotted onto solid yeast YPD medium supplemented without or
with additional AlCl
3
(6 mmol/L, 6.5 mmol/L and 7 mmol/L),
growth were monitored for 3~6 d at 3
0℃
Figure 4 Growth assay of yeast expressing
PutSTE24
and
AtSTE24
in the different stresses
Note: Yeast cells containing pAUR123, pAUR123
-
PutSTE24
and pAUR123
-
AtSTE24 were, respectively, incubated as
described in materials and methods; Serial dilutions were
spotted onto solid yeast YPD medium supplemented without or
with additional stresses, such as low pH (pH values 4.2),
sorbitol 1.6 mol/L, NaCl 0.9 mol/L and H
2
O
2
4.8 mmol/L,
growth were monitored for 3~6 d at 3
0℃