Basic HTML Version
Molecular Soil Biology (online), 2011, Vol. 2 No.2, 9
-
14
ISSN1925-2005
http://msb.sophiapublisher.com
- 11 -
Figure 1 Nucleotide sequences and the encoded amino acid sequences of
PutSTE24
Al
3+
stress can also cause some other stresses at the
same time, such as low pH and oxidation stresses,
therefore, in this study, growth of these transformed
yeast lines was observed in the conditions of pH 4.2,
sorbitol, NaCl and H
2
O
2
(Figure 4). The growth of the
PutSTE24
and
AtSTE24
transformants was the same
as that of the control in the presence of low pH and
sorbitol, but was better than that of the control on the
media containing NaCl and H
2
O
2
. The results indicate
that STE24 protease plays a role in response to salt
and oxidation stresses and its role in Al
3+
tolerance
may be not specific.
1.3 Responsion of
PutSTE24
and
AtSTE24
over-
expressing cells to various of metal cations
To further discuss the responsive relationship of
STE24
with metal ions except Al
3+
, serial dilutions
were spotted onto solid yeast YPD medium
supplemented without or with various of metal cations
and the growth was monitored (Figure 5). As shown in
Figure 5, the growth of the two
STE24
transformants
was much better than that of the empty vector
transformant on the media containing K
+
, Mg
2+
and
Cu
2+
; some better than the control with the Fe
3+
and
Cd
2+
; and was almost the same as that of the control in
the presence of Ca
2+
, Mn
2+
and Ba
2+
. Interestingly, the