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Molecular Plant Breeding 2012, Vol.3, No.3, 26
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performed on the 3730xl DNA analyzer (Applied
Biosystems). DNA sequence chromatograms were
carried out using the phred software. Subsequently,
sequences were vector-trimmed using Cross_Match
software in the phrap package and quality-trimmed
according to the quality value determined by phred.
The final (processed?) sequences were submitted to
the GenBank dbEST database. To identify unigenes,
phrap was used to assemble ESTs into contigs with the
parameters of 38 bp overlap length and 96% overlap
identity.
3.6 Functional annotation of ESTs and unigenes
For annotation of the ESTs and unigenes, sequences
were compared using BLASTX against both non-
redundant protein database and non-redundant nucleic
database at the NCBI site. Functional annotations
were based upon searches against multiple databases
in order to identify putative function of ESTs and
unigenes. According to Gene ontology (GO) classi-
fication and BLASTX comparison, the sequence
data were divided into different functional groups
characterized according to putative and potential roles
in berry development and compositions.
This study was supported by China Agriculture
Research System (CARS
-
30).
Authors' Contributions
XNJ and BL planned and conducted experiments, analysed the
data and wrote the first draft of the manuscript. WZ and CJY
helped on a regular basis in data analysis. JW conceived the
idea of the experiments and modified the manuscript. All
authors read and approved the final manuscript.
Acknowledgements
This study was supported by China Agriculture Research
System (CARS
-
30).
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