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Molecular Plant Breeding 2012, Vol.3, No.2, 11
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polymorphism between the two species (Supplementary
Table 1). Out of a total 596 alleles, 179 alleles were
unique to
C. capsularis
and 267 alleles were unique to
C. olitorius
. The PIC per SSR locus calculated over all
SSR loci did not differ much in the two species (0.198
vs 0.203; Table 1).
All the 172 polymorphic SSR loci used in the present
study were amplified in
C. olitorius
, but cross-species
amplification of only 156 SSRs (90.69%) showing
expected size of fragments was observed within
C.
capsularis
(Supplementary Table 1). In
C. capsularis
,
a set of 112 loci were polymorphic carrying 286
alleles (2.55 alleles per locus) and in
C. olitorius
, 140
loci were polymorphic carrying 385 alleles (2.75
alleles per locus) (Table 1; Table 2; Figure 1). The list
of polymorphic loci with range and mean of PIC
values are presented in Table 2. A fairly large fraction
of the polymorphic SSR loci were unique (24 loci in
C.
capsularis
and 52 loci in
C. olitorius
) to each of the
two species of jute (Table 2).
Figure 1 A representative figure showing polymorphism due to
(A) SSR MJM212 in
C. capsularis
and (B) SSR MJM609 in
C.
olitorius
Note: Lanes marked with “N” indicate null alleles; Lanes: 1 =
CIM003, 2 = CIM048, 3 = CIM013, 4 = CIM020, 5 = CIM014,
6 = CIM055, 7 = CIM024, 8 = OIM, 9 = OIM, 10 = CIM, 11 =
CIM, 12 = OIM037, 13 = OIM013, 14 = OIM019, 15 = OIM038,
16 = OIM011, 17 = OIM039, 18 = OIM029, 19 = OIJ243; M =
100 bp Ladder
1.2 Rare alleles
Out of 172 SSR loci, 118 loci had 157 rare alleles, each
allele having a frequency of <5% (Supplementary
Table 1). Out of 157 rare alleles, 25 were available in
both the species; 59 rare alleles were unique to
C.
capsularis
and 73 rare alleles were unique to
C.