Molecular Plant Breeding 2011, Vol.2, No.9, 60
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Figure 1 Phylogenetic tree derived from the full amino acid
sequences of starch synthesis proteins
Note: The tree was drawn according to the results generated by
PhyML Online analysis (http://mobyle.pasteur.fr/cgibin/Mobyle
Portal/portal.py?form=phyml) with the JTT model. Bootstrap
values calculated for 100 replicates are indicated at
corresponding nodes. The scale bar represents the branch
length corresponding to the indicated substitutions per site. The
GenBank accession numbers are shown in brackets
syntenic region of rice GFb in sorghum is 248 kb on
sorghum chromosome 9 (sC9). In sorghum, the GFa
contains 58 putative protein-coding genes (from
Sb03g032810, a Ras-related gene, to Sb03g033370,
an Ole e I family gene), while the GFb contains 36
putative protein-coding genes (from Sb09g026510, an
Ole e I family gene, to Sb09g026820, a Ras-related
gene) (http://rapdb.dna.affrc.go.jp/viewer/ gbrowse/
Figure 2 Comparison of the starch synthesis genes on the rice/
sorghum/maize synteny physical map
note: rC=Rice chromosome, sC=sorghum chromosome, mC=
maize chromosome. Arrows indicate the loci of
SS
Ⅳ
genes
build4/) (supplement and Figure 2). GFa and GFb
have 12 paralogous genes in sorghum. There are 44
orthologous genes in GFa, while 29 orthologous genes
in GFb between in the rice genome and sorghum
genome. Like the
OsSS
Ⅳ
b
in rice, the
SbSS
Ⅳ
b
gene
was also located between a serine acetyltransferase 1
and a NADH-ubiquinone oxidoreductase 20 kD
subunit in sorghum. However, the
SS
Ⅳ
a
gene which
located between the chlorophyll a-b binding protein
gene and serine acetyltransferase gene in GFa in rice
was lost in GFa in sorghum (supplement and Figure 2).
Based on the sorghum-maize synteny (http://www.gra