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Molecular Plant Breeding 2011, Vol.2, No.9, 60
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Table 1 Domain organization of the SS proteins in Gramineae
Gene name
Protein domains
Sorghum
Rice
Maize
Wheat
GBSSI
TP
77
76
72
71
VR
4
5
4
6
CD
526
526
530
528
GBSSII
TP
76
77
76
67
VR
4
3
4
4
CD
527
528
530
528
SSIIa
TP
61
70
62
16
VR
190
248
178
291
CD
492
492
486
492
SSIIb
TP
28
64
29
63
VR
184
138
177
119
CD
492
492
492
492
SSIIc
TP
64
44
69
64
VR
209
193
214
186
CD
492
492
492
492
SSIIIa
TP
68
49
69
67
VR
668
558
657
611
SBD
490
488
492
490
CD
448
444
448
458
SSIIIb
TP
81
47
47
45
VR
181
222
200
308
SBD
471
489
486
460
CD
448
448
448
444
SSIVa
TP
78
VR
399
CD
485
SSIVb
TP
54
33
54
45
VR
361
388
361
375
CD
484
484
485
486
Note: TP: The putative transit peptide, which was identified using the ChloroP neural network analysis of the 100 amino acids at the
N-terminus of each sequence; CD: The putative catalytic domain, which was identified by GenBank conserved domain database
search service, v2.13; VR: The variable region; SBD: The starch binding domain
The selected genomic fragment containing the
SS
Ⅳ
a
gene (GFa) was 544 Kb on rice chromosome 1 (rC1)
which contains 63 putative protein-coding genes
(from Os01g0714900, a Ras-related gene, to Os01g0-
726100, an Ole e
Ⅰ
family gene). The genomic
fragment containing the
SS
Ⅳ
b
gene (GFb) was 296 Kb
on rice chromosome 5 (rC5) which contains 34
putative protein-coding genes (from Os05g0531200,
an Ole e I family gene, to Os05g0536900, a Ras-
related gene) (http://rapdb.dna.affrc.go.jp/viewer/gbr-
owse/build4/) (supplement and Figure 2). There are 16
paralogous between GFa and GFb. The
OsSS
Ⅳ
a
gene
is located between a chlorophyll a-b binding protein
gene and a serine acetyltransferase gene, while the
OsSS
Ⅳ
b
gene located between a serine acetyltrans-
ferase gene and a NADH-ubiquinone oxidoreductase
20 kD subunit gene (supplement and Figure 2).
Based on the rice-sorghum synteny (http://www.gram-
ene.org/), the syntenic region of rice GFa in sorghum
is 436 kb on sorghum chromosome 3 (sC3), while the