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Molecular Plant Breeding 2011, Vol.2, No.9, 60
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Table 2 Primers used for sorghum in this study
Primer name
Sequence (5′→3′)
Annealing temperature (
℃
)
cDNA cloning
sGBSSIIF
AGCACTAGCAGCCGCGAGGTCATC
60
sGBSSIIR
AACTGGTGGTTTTGCCGGTGTCTG
sSSIIaF
ATGTCGTCGGCGGCCGTGTCGTCC
65
sSSIIaR
CCTCTGTCAACGCACCAACCCCCAGC
sSSIIbF
AAGCAGCAGCAGCAGTAGCGT
63
sSSIIbR
AGGCATCACCGTAGCTCACATACA
sSSIIcF
CTCCAATGGCGGCTTCTACTTTC
60
sSSIIcR
CCTCCTGTACCAAAAGCGTCTAA
sSSIIIaF1
TCTATAAATGGAGATGGCCCTACG
60
sSSIIIaR1
AGCAAGCTCTTGAAGCATCCTCTG
sSSIIIaF2
GGAGTCACCTAATGATAACGTGGA
60
sSSIIIaR2
CTAACTGAAGACAAGGAGAGGCTG
sSSIIIbF1
GGGCTGAGGGGGGAGGTTTTGC
60
sSSIIIbR1
CTTCCCATAGACACATCCAACCCA
sSSIIIbF2
TGGGTTGGATGTGTCTATGGGAAG
60
sSSIIIbR2
AGAACTGGTCCGAACTCCGAAGTC
Semi-RT-PCR
SbGBSSIF
TGACCACCCGCTGTTCCTTGAG
56
SbGBSSIR
ACGGCGATGTACTTGTCCTTGC
SbGBSSIIF
CAACAACGGCACCTGAAGCAAG
58
SbGBSSIIR
CTCAACATCTCGCCAGCCACC
SbSSIF
GATGGGTTGGATTTAGTGTTC
52
SbSSIR
CACGTGAAGTCTTTTGACATG
SbSSIIaF
ACCCAAGGCTTTAGCGAGAAG
56
SbSSIIaR
CAGGAGTGCAGTGTGCCAATC
SbSSIIbF
AGAAGCCCGAGATTTAGGTGTG
56
SbSSIIbR
CACGACCCTGATGAGCAATG
SbSSIIcF
GACACCACTTACGGAGAATC
58
SbSSIIcR
GATGTCAGTTCGGTTCCCAC
SbSSIIIaF
TTATTTGAGGATTTCTTGGCTG
58
SbSSIIIaR
ATGTTATTCGGAAGGGTAGC
SbSSIIIbF
CTACATTCCCAAGCAGGCATAC
56
SbSSIIIbR
TTTCAGCACCCTCCCTTTCTC
SbSSIVF
CAAGATATGGACTGGGACTGT
52
SbSSIVR
GCCACAATATGCTAAATCCTG
ActF
GGAACCGGTATGGTCAAGGC
60
ActR
AGTCTCGTGAATGCCAGCAG
Table 3 Primers used for wheat in this study
Primer name
Sequence (5′→3′)
Annealing temperature (
℃
)
cDNA cloning
tSSIIbR1
TAGTTCCAGTACGTGTTGAGGCAG
56
tSSIIbR2
AAGGCTCGAACAGCGACGGCATGA
tSSIIbF1
GGCCGTTCAAAGAGCATATC
55
tSSIIbF2
CATCCCATTCTGCGGTTCCA
tSSIIIbR1
TTCATCCCCGTCACCTTCTATATGC
52
tSSIIIbR2
AGAACACAAAGTCTAATCTGTATGC
tSSIIIbF1
TTGGGTTGGATGTGTCTATG
50
tSSIIIbF2
TCTCCCTGCCAGCTTAAAGTCT