Page 12 - Molecular Plant Breeding

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Molecular Plant Breeding 2011, Vol.2, No.16, 109
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Figure 5 Prediction of secondary structure among cloned SCAs and other species LTPs
Note: Blue: Alpha helix, Yellow: Random coil, Red: Extended strand, Green: Beta turn; Figures represent numbers of amino acid
residues
difference existing in different copies of the introns of
same species. Although intron does not encode protein,
it plays an important regulatory role in gene expression.
In most of cases, intron will enhance gene expression
(Callis et al., 1987), of which the enhanced degree of
gene expression would be related with many factors,
such as characteristics of intron (Clancy and Hannah,
2002), features of promoter and exon sequence, intron
positions on the vector (Wang, 2008), the sequence
structure of the target gene, cell type and cell
physiological status. Some introns have tissue-specific
regulation to gene expression (Stemmler et al., 2005),
while some introns have a disincentive function on
gene expression (Hormuzdi et al., 1998). In this study,