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Molecular Plant Breeding 2011, Vol.2, No.13, 92
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Figure 2 The position of QTLs for plant height in wheat
Note:
: Beijing 2007; : Fu’nan 2007; : Beijing
2008; : Fu’nan2008
Therefore, traits like plant height are subject to change
in environment, we should select larger group to
eliminate differences of the local environment, which
thus leads to the phenotype error and reduce to the
effects of molecular marker’s segregation distortion.
But a group too large undoubtedly increases the
workload, the number of groups range from 150 to
300 per plant (lines) (Zhang, 2002, Chinese Science
Bulletin, 51(9): 2223-2231).
In Beijing and Fu’nan, QTL for plant height on
chromosome 6B was detected in both years, the result
indicated this QTL was subject to little effect in
environment. At present, other researchers have not
yet found the relevant reports of the QTL, which may
be a new QTL loci controlling plant height. In this
study, we detected QTL for plant height on
chromosome 6A, with interval was from barc3 to
barc107. Results of the study are in line with the
works by Wang et al (2008) and Liu et al (2009),
QTLs were detected by them near Xgwm570, which is
closed to the barc3. we also detected a QTL in
barc163-gwm495 interval on the 4B chromosome, this
result is in line with the works by Liu et al (2009),
while Liu detected a QTL in Xgwm107.2-Xgwm113
interval, this result suggested that the same QTL has
been detected on the 4B chromosome. Stability and
credibility of detected QTL for genetic improvement
of plant height and marker-assisted breeding for dwarf
and semi-dwarf wheat.
Detection of different mapping population leads to the
discovery of more QTLs, and comprehensive grasp of
gene location in the chromosomes is the basis of
genetic research for traits. The QTL detection in
different groups may be relatively stable loci formed
in the long-term process of evolution, and value in use
of molecular marker-assisted selection.
3 Materials and Methods
3.1 Experimental materials
In this study, we obtained F
1
generation by crossing
sterile line BS366 and Baiyu149, which were used to
be as maternal and paternal materials, respectively,
and provided by Beijing Research Center for Hybrid
Wheat, Beijing Academy of Agriculture and Forestry
Sciences. 234 doubled haploids are derived from F
1
generation through anther culture and chromosome
doubling, and then formed a mapping population.
After years of observation and identification, BS366
has a stable photoperiod and temperature sensitive
genie male sterile characteristic.
3.2 Field experiment
In 2007~2008 and 2008~2009 year, the parents and
234 DH lines were grown in Haidian Experimental
Station of Beijing and Fu’nan Experimental Station of
Anhui, Random block design, two replications, one
row for each strain, 1.5 m length, 40 grains in each
row, and conventional management strategies in
grower fields. Plant height from the ground to top
excluding awns is counted by centimeters, and