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average height of effective stem is marked plant
height for the row.
3.3 Construction of linkage map
In this research, 1428 pairs of primers derived from
published sequences of Röder et al (1995), Smulders
et al (1997) and Gupta et al (2002), were synthesized
by SBS Genetech Co., Ltd.
Linkage analysis of marker results by using QGA_CN
software, which was provided Professor Zhu Jun in
Zhejiang University. According to the linkage distance
between markers and high-density microsatellite map
of wheat published by Somers et al (2004), we drew a
linkage map by using Mapdraw v2.1 software. 130
pairs of primers were marked on the chromosome 21
of wheat, the total length of linkage map was 1711.52
cM, the average genetic distance was 13.17 cM
between markers.
3.4 Statistical analysis
The basic statistical analysis for plant height is
performed by 6SQ software, and variance analysis is
performed by SPSS 16.0 software.
3.5 QTL analysis
We analyze QTLs of plant height in four kinds of
environmental conditions based on the method of
composite interval mapping (CIM) of WinQTLCart
2.5 software packages, which is developed by North
Carolina State University in USA, 2.5 LOD threshold
value. If LOD exceed 2.5 in marker interval, so one
QTL controlling trait was detected in this interval, and
calculated the contribution rate and additive effect of
each QTL.
Author Contributions
LPZ and XQX conceived the overall study, performed the experiment
designs, and drafted the manuscript. CPZ and FHS took part to the data
analysis and the writing. SHY and QX obtained and analyzed the data and
were involved in the writing. All authors read and approved the final
manuscript.
Acknowledgements
This work was supported by the Beijing Municipal Natural Science
Foundation (No. 5091001) , a grant from the National High Technology
Research and Development Program of China (863 Program) (No.
2011AA10A106), 948 Program (No.2009-Z4), and Innovation Platform
Program for Basic Research of Agricultural Breeding in Beijing (No.
D08070500690801). The authors thank the coconut farmers from the six
cities for their assistance.
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