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Molecular Plant Breeding 2011, Vol.2, No.10, 68
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74
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69
wheat grains. The objectives of the present study were
to identify the QTLs controlling water-soluble oligosa
ccharides and clarify the relationships among the
QTLs using a population of recombinant inbred lines
(RIL) derived from two Chinese winter wheat
varieties. The results may be used by breeders in
marker-assisted selection for crop improvement.
1 Results and Analysis
1.1 Phenotypic variations and correlations between
traits
Five types of water-soluble saccharides (glucose,
fructose, sucrose, maltose, and raffinose) were
detected by high-performance liquid chromatography
(HPLC) (Figure 1, Table 1). The two parents, Chuan
35050 and Shannong483, differed significantly in
their water-soluble saccharide contents (Table 1).
Chuan 35050 had higher sucrose and raffinose
contents than Shannong 483, while Shannong483 had
higher fructose, glucose, and maltose contents than
Chuan 35050. The RIL population showed wide
variations in the contents of all water-soluble
saccharides. The coefficient of variation (CV) for
raffinose, sucrose, glucose, fructose, and maltose
content was 48.48
%
, 42.50
%
, 36.97
%
, 35.31
%
, and
33.09%, respectively.
Simple correlation coefficients (r) between pairs of
water-soluble saccharides are listed in Table 2. The
highest simple correlation coefficient was between
glucose content and fructose content (0.848).
Raffinose content was significantly negatively
correlated with glucose content and fructose content.
The relationships between the other pairs of water-
soluble saccharides were all non-significant at 0.05
and 0.01 levels.
Figure 1 Chromatogram of water-soluble saccharides separated
by high performance liquid chromatography (HPLC)
Note: 1: Fructose; 2: Glucose; 3: Sucrose; 4: Maltose; 5:
Raffinose
Table 1 Water-soluble saccharide contents of RILs and their parents
Parents
RIL population
Water-soluble saccharide
contents (10
-
4
g/mL)
Chuan35050
Shannong483
Average
Min
Max
SD
CV (%)
Glucose content
2.67
6.28
3.73
2.02
10.81
1.38
36.97
Fructose content
1.30
1.82
1.38
0.54
2.98
0.49
35.31
Sucrose content
0.75
0.36
0.59
0.25
1.77
0.25
42.50
Maltose content
1.35
3.53
1.28
0.27
2.84
0.42
33.09
Raffinose content
0.59
0.33
0.41
0.03
1.11
0.20
48.48
Table 2 Simple correlation coefficients (r) between pairs of water-soluble saccharides
Water-soluble sacch-
aride contents
Glucose content
Fructose content
Sucrose content
Maltose content
Fructose content
0.848**
Sucrose content
-
0.154
-
0.163
Maltose content
0.098
0.009
0.097
Raffinose content
-
0.266**
-
0.184*
0.139
-
0.164
Note:
“*” and “**” mean statistical significant at the 0.05 level and 0.01 level respectively
1.2 QTL analysis
A total of ten putative QTLs for three water-soluble
oligosaccharide contents (sucrose, maltose, and
raffinose content) were located on nine chromosomes
that assign to chromosome of 1B, 1D, 2B, 2D, 3B, 4A,
5A, 5D, and 6B. The ability of a single QTL to