Basic HTML Version
Molecular Plant Breeding 2011, Vol.2, No.10, 68
-
74
http://mpb.sophiapublisher.com
68
Research Letter Open Access
QTL Detection for Water-soluble Oligosaccharide Content of Grain in Common
Wheat
Xiyang Fu , Zhaoliang Qi , Sishen Li
Agronomy College of Shandong Agriculture University, State Key Laboratory of Crop Biology, Tai’an, 271018
Corresponding author email:
ychli@sdau.edu.cn;
Author
Molecular Plant Breeding, 2011, Vol.2 No.10 doi: 10.5376/mpb.2011.02.0010
Received: 30, Mar., 2011
Accepted: 14, Apr., 2011
Published: 22, Jun., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Fu et al., 2011, QTL Detection for Water-soluble Oligosaccharide Content of Grain in Common Wheat, Molecular Plant Breeding Vol.2 No.10 (doi:
10.5376/mpb.2011.02.0010)
Abstract
In present study, 131 recombinant inbred lines (RIL) derived from a cross between Chuan 35050 and Shannong 483 were
used to detect the quantitative trait loci (QTL) conferring to water-soluble oligosaccharide content of grain in common wheat
(
Triticum aestivum
L.). Ten additive QTLs for water-soluble oligosaccharide content of grain were identified and mapped on nine
chromosomes that assigned to chromosome of 1B, 1D, 2B, 2D, 3B, 4A, 5A, 5D, and 6B, respectively. Four of the detected QTLs
were conferred to sucrose content, another four QTLs were assigned to maltose content, and other two QTLs to raffinose content.
The phenotypic variations of the single detected QTL can be explained from 6.98% to 38.30%. The further analysis indicated that
five QTLs were identified with positive additive effects contributed by Chuan35050, whereas the additive effects of the remaining
five QTLs were negative by contribution of Shannong 483. In this research, we detected a QTL named
qMac
-
2D
-
1
on chromosome
2D conferring maltose content of wheat grain that can explain 38.30% of phenotypic variance, which might imply that the
qMac
-
2D
-
1
be a major QTL with the increasing effect from the parent of Shannong 483.
Keywords
Common Wheat (
Triticum aestivum
L.); Quantitative trait loci (QTL); Oligosaccharide; Sucrose; Maltose; Raffinose
Background
Oligosaccharides are carbohydrates composed of three
to ten monosaccharides joined via glycosidic linkages.
They can be classified as digestible or functional,
according to their digestion and absorption by animals.
Digestible oligosaccharides like sucrose, lactose,
cyclodextrin, and maltose, are readily digested by
enzymes in the human gut and provide energy.
Functional oligosaccharides, such as stachyose and
raffinose, are neither digested nor absorbed in the
human stomach and intestine (Kaneko et al., 1995).
These functional oligosaccharides are considered to be
prebiotics that promote the growth of colonic bifid
bacteria and
Lactobacillus acidophilus
, thereby
improving human gut health.
Oligosaccharides are naturally present in various
grains. Wheat (
Triticum aestivum
L., 2n=42, AABBDD
genomes), which is one of the major food crops
worldwide, contains a number of oligosaccharides in
its grains, including sucrose, maltose, and raffinose. In
wheat grains, oligosaccharides are significantly less
abundant than starch and protein, and they are
complicated quantitative traits. Quantitative trait locus
(QTL) analysis has provided an effective approach to
dissect complicated traits into component loci to study
their relative effects on a specific trait (Doerge, 2002).
Many QTLs for oligosaccharide content and polysac
charide content have been detected in some crops by
using recombinant inbred lines (RILs), double haploid
(DH) lines, or other populations, combined with
genetic linkage maps. QTL analyses have been used to
examine several polysaccharides, for example, barley
fructan (Hayes et al., 1993) and β
-
glucan (Han et al.,
1997; Igartua et al., 2000; Han et al., 2004), oat β
-
gl
ucan (de Koeyer et al., 2004), and soybean oligosacch
arides (sucrose, raffinose and stachyose) (Hyeun et al.,
2006; Cicek et al., 2006) .
Although quality traits in wheat have been studied
using QTL analyses, such analyses have not been used
to examine water-soluble oligosaccharide contents in