Page 6 - Molecular Plant Breeding

Liu et al 2008, Genetic Diversity of
Involved Varieties and Improvement of Elite Restorer of Indica Rice (Oryza sativa L.) Using Backcross Introgression, Molecular Plant Breeding, 6(1):25-31
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yield, good quality, permanent resistance to rice blast
and all kinds of biotic and abiotic stresses in different
places, including insect, drought and cold stresses,
high N,P,K use efficiency, lodging resistence in
Yangtze river. Conventional methods with limited
parents hybridization cannot satisfied high requirement
of modern agricultural industry due to the long period
and low efficiency.
A strategy, integrates germplasms’ efficient utilization,
exploration of favorable genes with different target
characters and new cultivar development, has been
brought forward in the program of global molecular
rice breeding by Dr Li. (Li et al., 2005; Ali et al., 2006;
Lafitte et al., 2006). Using germplasms from all over
the world, hybridizations are conducted to widely
planted cultivars in local area. Then screening for
target characters is carried out after 2~3 times
backcrosses and one self-cross. Consequently,
backcross introgression of favorable QTL with target
characters from different cultivars will be obtained
and exploration of gene/QTL will closely be combined
with genetic breeding.
In this research, 95 pairs of SSR primer in rice
chromosome were selected for preliminary screening
and 53 pairs were chosen in view of good amplifycation
and clear banding. Genetic diversity anrelationship
among different cultivars were investigated, by means
of PCR amplification to 55 copieof rice originated
from different sources, to make reference for large-scale
exploration and efficient utilization of favorable gene.
1 Materials and Methods
1.1 Materials
In our study, 55 accessions of rice, originated from
major rice growing regions around the world are used.
These materials include 36 accessions from China, 4
from Philippines, 4 from International Rice Research
Institute, 3 from India, 2 from Iran, 1 from America,
from Japan, from Malaysia, 1 from Nepal, 1 from
SriLanka and 1 from Indonesia (Table 1).
Table 1 Materials used in present study
Series number Variety name Origin Subspecies a Series number Variety name Origin Subspecies a
1 Minghui86 China i 29 Pusa India i
2 Shuhui527 China i 30 Basmati India i
3 9311 China i 31 IRAT352 Indonesia i/j
4 Minghui63 China i 32 Khazar Iran i
5 Fuhui838 China i 33 Domsiah Iran i/j
6 Gui99 China i 34 IR64 IRRI i
7 Fengaizhan China i 35 Teqing China i
8 Zhong413 China i 36 IR24 IRRI i
9 Zihui100 China i 37 IR65600-27-1 -2-2 IRRI i
10 Aizidao China i 38 FUJISAKA5 Japan i
11 Diantun502 China i 39 MR167 Malaysia i
12 Jiangxisimiao China i 40 Pokhreli Nepal i/j
13 Mengguandamagu China i 41 PSB RC 66 Philippines i
14 Yunhui72 China i 42 Milagrosa, Zawa Banday Philippines i/j
15 Yetuosui China i 43 IR6 IRRI i
16 ZDZ057 China i 44 BJ1 Philippines i/j
17 Luhui17 China i 45 Bg90 -2 Sri Lanka i
18 Hanhui10 China i 46 Lemont USA i
19 Han3 China i 47 2818 China i
20 Han4 China i 48 3008 China i
21 Han5 China i 49 9014 China i
22 Han297 China i 50 2004 China i
23 Han277 China i 51 829 China i
24 Dianjing China i 52 3750 China i
25 Wangyangzhan China i 53 Duonieai China i
26 Hangxiang10 China i 54 XL1018/XL998-1 Xuanxi China i
27 Dali China i 55 P88 Philippines i
28 TKM9 India i
Note: a: i denoted indica; j denoted japonica; i/j denoted the medium type of indica and japonica