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Molecular Plant Breeding 2011, Vol.2, No.1, 1
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Research Report Open Access
Genetic Diversity of Involved Varieties and Improvement of Elite Restorer of
Indica Rice (
Oryza sativa
L.) Using Backcross Introgression
Jinteng Cui
1
, Bingxu Chen
2
, Yingyao Shi
3
, Rong Zhang
4
, Hui Wang
2
, Yiliang Qian
3
, Haiyan Liu
2
,
Linghua Zhu
2
, Zhikang Li
2
, Yongming Gao
2
1. Landscape College, Beijing University of Agriculture, Beijing, 102206, P.R China
2. Institute of Crop Sciences, Chinese Academy of Agricultural Science, Beijing, 100081, P.R China
3. College of Agricultural Sciences, Anhui Agricultural University, Hefei, 230036, P.R China
4. Feed Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081, P.R China
Corresponding author email:
gaoym@caas.net.cn;
Authors
Molecular Plant Breeding, 2011, Vol.1, No.1 doi: 10.5376/mpb.2011.02.0001
Received: 26, Nov., 2010
Accepted: 20, Dec., 2010
Published: 18, Jan., 2011
This article was first published in Molecular Plant Breeding (Regular Print Version) in Chinese, and here was authorized to redistribute in English under the terms of the Creative
Commons Attribution License , which permits unrestricted use, distribution, and reproduction in anymedium, provided the original work is properly cited.
Preferred citation for this article:
Liu et al., 2008, Genetic diversity of involved varieties and improvement of flite restorer of indica rice (
Oryza sativa
L.) using backcross introgression, Molecular Plant Breeding,
6(1):25-31 (doi:10.5376/mpb.2011.02.0001)
Abstract
To make reference to cross combinations and improvement of parents, the genetic diversity and relationship among 55
rice germplasms were analyzed with a total of 53 SSR markers. In the present study, 267 allelic variations were detected, and the
average allelic variation of 53 loci was 5.04, ranging from 4 to 7. The average polymorphism index content (PIC) of SSR markers
was 0.624, ranging from 0.287 to 0.786. All germplasms could be divided into indica and japonica rices, the genetic similarity
between them varied from 0.588 to 0.996. The similarity coefficient between Minghui 86 and 53 donors was from 0.655 to 0.850.
The similarity coefficient between Shuhui527 and the donors was from 0.640 to 0.873. The results indicated that the detection of SSR
polymorphisms was not only one of the most efficient and accurate measures to study the genetic differences among rice varieties but
also was helpful for the discovery and utilization of favorable genes in advanced-backcross introgression populations.
Keywords
Rice; SSR; Genetic diversity; Similarity coefficient
Background
The success in hybrid rice is another great breakthrough
following dwarf breeding in rice industry in China.
Recently, the percentage of hybrid rice combination in
hybrid and inbred regional tests in southern growing
areas is increasing progressively. In mid indica rice,
late indica rice and Huanan late indica rice groups,
this percent has arrived more than 90% or even 100%
in 2004. Besides, the percentage of early indica rice
and Huanan early indica rice groups is over 80% and
exceed 60% in late japonica rice group (Yang et al.,
2004). Nowadays, the objective of hybrid rice
breeding is far more than high yield. On condition of
high production character, much attention has been
focus on good quality, desirable resistance to
multi-biotic or abiotic stresses and nutriment
efficiency. The improvement of parents in hybrid rice
is of great importance to achieve all of these goals.
Both Minghui86, bred by Sanming Academy of
agricultural science in Fujian province in 1994, and
Shuhui527, bred by Rice Institute in Sichuan
Agricultural University, are elite restorer lines in
recent 10 years. Gangyou527 and Dyou527, which use
Shuhui527 as an essential restorer line, are developed
and popularized to 715 and 439 million mu, respectively,
ranking the sixth and eighth in 2004. In addition, II
Minghui86, a combination between Minghui86 and
II-32, was planted to 369 million mu, which got the
tenth place in 2004 (National Agro-Tech Extension
and Service Center (NATESC) 2005; Statistics table
of major cultivars’ extention status of National Crop
in 2004).
The span of rice growing region in China is very large,
therefore hybridization requirements differ a lot due to
the diversity of environment and breeding objectives.
A desirable hybrid rice for extension should have both
excellent characters and broad adaptability. At present,
the objective of hybrid rice breeding include: high