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Molecular Plant Breeding 2010, Vol.1 No.4
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Research Article Open Access
Molecular Tagging Wheat Powdery Mildew Resistance Gene
Pm21
by EST-SSR
and STS Markers
Xiaolei Qi
1
, Fa Cui
1
, Li Yu
1
, Anming Ding
1
, Jun Li
1
, Guiling Chen
1
, Honggang Wang
1,2
1.State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018
2.Tai’an Subcenter of National Wheat Improvement Center, Agronomy College of Shandong Agricultural University, Tai’an, 271018
Corresponding author email: hgwang@sdau.edu.cn;
Authors
Molecular Plant Breeding 2010, Vol.1 No.4 DOI:10.5376/mpb.2010.01.0004
Received: 6 May, 2010
Accepted: 10 June, 2010
Published: 28 September, 2010
This is an Open Access article distributed under the terms of th
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original work is properly cited.
Preferred citation for this article:
Qi et al 2010, Molecular Tagging Wheat Powdery Mildew Resistance Gene
Pm21
by EST-SSR and STS Markers, Molecular Plant Breeding Vol.1 No.4 (DOI:
10.5376/mpb.2010.01.0004)
Abstract
In order to enrich the molecular markers linked to wheat powdery mildew resistance gene
Pm21
and facilitate the
molecular marker-assisted selection in multi-background breeding procedure, preferred small groups (PSG) analysis was conducted
using
F
2
segregating population cross between the wheat germplasm line CB033 with
Pm21
and a common wheat variety
Huixianhong (susceptible to wheat powdery mildew). One hundred and two markers revealed polymorphism between the parents
when screening with 258 SSR or STS markers located on wheat homoeologous group VI, and two EST-SSR markers (
Xcfe164
and
Xedm129
) and one STS marker (
Xcinau188
) showed good consistency between the genotype and the phenotype to mildew in PSG.
Linkage relationship between
Pm21
and molecular markers of
Xcfe164
,
Xedm129
and
Xcinau188
were then established, with the
distance 3.75 cM, 1.26 cM and 0.98 cM respectively. Authenticity verification of the linkage between
Pm21
and molecular markers
were tested via F
2: 3
families and results showed that the three markers were reliable new markers linked to
Pm21
. The results above
would facilitate molecular marker-assisted selection in breeding program.
Keywords
Pm21
; Preferred small group (PSG); Molecular tagging; EST-SSR; STS
Background
Wheat powdery mildew caused by the obligate
biotrophic fungus
Erysiphe
(
Blumeria
)
graminis
f.sp
triticale
, is one of the most serious wheat diseases,
which lead to grain weight reduced, also play an
negative influence on grain quality. With the
promotion of semi-dwarf wheat varieties and the
increasing levels of Nitrogen fertilization, the
powdery mildew was more popular in China, and had
become a major obstacle for wheat production (Wand
et al., 2001). Identification and utilization of new
powdery mildew resistance genes in breeding, have
proven to be an effective means to protect the wheat
from powdery mildew. The gene
Pm21
located on the
short arm of 6VS from
Haynaldia villosa
, was one of
the most effective resistance genes, which conferred
resistance to all races of
B. graminis f. sp. tritic
in
China (Chen xiao et al. 1997), Europe (HUANG et al.,
1997), and The powdery mildew resistance gene
Pm21
has been successfully transferred to common
wheat, and located on the short arm of 6VS/6AL, (Qi
et al.1995). As the fact, the translocation lines showed
no obvious undesirable effects on agronomic and
quality traints (CHEN et al.1995, Li et al. 2007).
Up to now, the reported molecular markers for
Pm21
included RAPD, AFLP, SCAR, and STS markers. Qi
et al. (1996) found the RAPD markers
OPH17
-
1400
which could be tracked the gene
Pm21
, then Liu et al.
(1999) converted it to SCAR (sequence characterized
applied region) markers
SCAR
-
1400
and
SCAR
-
1265
;
Li et al. (2005) carried out RAPD analysis to the
6D/6V Substitution Pm930640 using 120 random pri-
mers to, and got 5 specific markers of 6VS; Cao et al.
(2006) developed a codominant marker
NAU/XiBao15-
-
902
(
CINAU15
-
902
) to identify 6VS according to
the powdery mildew resistance gene sequences; Chen
et al. (2006) also developed to a codominant marker
NAU/XiBao16
-
1085
(
CINAU16
-
1085
) to identify 6VS
based on the wheat LRR (leucine-rich repeat) sequence;