Basic HTML Version
Molecular Pathogens (online), 2011, Vol. 2
ISSN 1925-1998
http://mp.sophiapublisher.com
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Table 2 Positive carrying rate of
Salmonella
by biochemic test
Sampling locations
Carrying rate A group (%)
Carrying rate B group (%)
Total Carring rate (%)
Nandujiang
75.00
50.00
53.85
Wanquanhe
66.67
56.00
58.82
Haikoudonghu
42.85
-
50.00
Carrying rate of group
60.00
53.19
54.41
Note: A group: Carapace length of a red-eared slider less than 4
-
inch; B group: Carapace length of a red-eared slider equal or more
than 4
-
inch; “
-
”stands no statistical analysis with small sample size; Carrying rate = individuals of red-eared slider carrying
Salmonella
divided by total tested red-eared slider
1.3 Identification of
Salmonella
serotypes
The employed
Salmonella
diagnostic serum kit
(Model 60) produced by Lanzhou Institute of
Biological Products can theoretically detect
Salmonella
OA-F serotypes, while
Salmonella
diagnostic kits
produced by Thailand S & A company can detect OA-I
serotypes. The results showed that the two sampling
points of Nandujiang and Wanquanhe detected out
Salmonella common serotypes as
S. litchfiled, S.
chailey, S. senftenberg
, and
S. Stanley.
We were
unable to detect out any Salmonella common
serotypes
in samples of
Salmonella
isolated from Haikoudonghu,
which indicated that the
Salmonella
serotypes of
isolates from red-eared sliders in Haikoudonghu
should be outside of the A-F group (Table 3).
2 Discussions
According to the literature, 14% of human
Salmonella
infections in is caused by the spread of turtles (Cohen
et al., 1980), Richard et al (2004) detected 0% of wild
turtles carrying
Salmonella
. Saelinger et al (2006)
detected less than 5% of wild turtles carrying
Salmonella
, Vila et al (2007) detected 12% of overall
detection rate of
Salmonella
in one
kind of upland tortoises and two species of freshwater
turtles, Gaertner (2008) detected
Salmonella
in a
variety of wild freshwater turtles that the total carrier
rate was 51%, of which the wild red-eared sliders
carried
Salmonella
rate of 38%. The results of this
study for the total rate of wild red-eared sliders
carrying
Salmonella
was 54.41% (n = 68).
There is a little difference of
Salmonella
carrying rate
among three different sampling locations, it indicated
that habitation should have no effect on the carrying
rate of
Salmonella
, which was consistent with
Gaertner’s report (Gaertner, 2007). Nandujiang is a
kind of brackish water river, where
Salmonella
in the
sample was detected out, indicating that
Salmonella
as
less salt tolerant baterium, can be carried by
amphibians reptiles living in these aquatic
environment,
Salmonella
detection rate (50.00%) of
red-eared sliders from semi-artificial Lake of
Haikoudonghu was lower than that of the natural
rivers, which does not match the
Salmonella
carring
rate of turtles in the artificial environment reported in
the previous studies (Keymer, 1972). The reason
might be due to regularly release bleaching powder,
Table 3 Serotypes detection of
Salmonella
isolates in collected red-eared sliders
Locations
Serotypes
Name of S
almonella
isolates
No of strains
Serotypic groups
Nandujiang
O6,8:Hz4,z23:e,n,z15
S. chailey
2
C2
O6,8:He,h:1,2
S. newport
1
C2
O6,8:Hl,v:1,2
S. litchfield
1
C2
O4,5:He,h:
-
To be comfirmed
1
B
OA-F
To be serotyped
2
-
Wanquanhe
O4,12:Hd:1,2
S. stanley
1
B
O1,3,19:Hg,t:-
S. senftenberg
1
E4
O4,5:He,h:-
To be comfirmed
1
B
OA-F
To be serotyped
3
-
Haikoudonghu
No detected out
Note:
Salmonella
serotypes of isolates from red-eared sliders in Haikoudonghu should be outside of the A-F group