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Molecular Pathogens (online), 2011, Vol. 2
ISSN 1925-1998
http://mp.sophiapublisher.com
- 29 -
have intruded on Hainan Island, populations have
been found in red-eared sliders across the island, in
order to know the impacts of alien species, red-eared
sliders, on island ecosystems, especially on potential
harms of environment and human in the cayying and
transmiting of
Salmonella
. We collected samples of
red-eared sliders in Nandu river, Wanquan river on
Hainan Island, Donghu Lake in downtown of Haikou
city of Hainan to isolate
Salmonella
bacteria from
cloaca of red-eared sliders, in order to make clear the
status of the wild populations of red-eared sliders
carrying
Salmonella
.
1 Results and Analysis
1.1 Red-eared sliders collection and
Salmonella
identification
We collected 68 of wild red-eared sliders in
Nandujiang (Nandu river), Wanquanhe (Wanquan
river) and Haikoudonghu (East Lake of Haikou City)
during the period between Feb. to Sep. 2010 (Figure 1),
of which Nandujiang was 26, Wanquanhe was 34 and
Haikoudonghu was 8. Total tentative 98
Salmonella
isolates were obtained from 68 samples of red-eared
sliders. Further biochemical identification showed that
79
Salmonella
bacteria were detected out of Total
tentative 98
Salmonella
isolates, of which 37
red-eared sliders carried
Salmonella
, Total carrying
rate of
Salmonella
in collected red-eared sliders was
54.41%, of which Nandujiang was 53.85%,
Wanquanhe was 58.82%, Haikoudonghu was 50%.
1.2 Biochemical identification of
Salmonella
In this study we used
Salmonella
biochemical
identification kit produced by Huankai Biotechnology
Figure 1 Selected photos of Red-eared sliders (
Trachemys
scripta elegans
) collected in Hainan island
Co., Ltd. Guangdong to determine serological
reactions of
Salmonell
a. The employed Kit contained
three response categories (A1, A2 and A3) with six
standard identifications of
Salmonella
(Table 1). The
results showed that 24 individuals of red-eared sliders
response to A1
-
1 reaction.
According to biochemical test positive data, further
statistics showed that the tested sample of 68
red-eared sliders had a total carrying rate of 54.41%,
of which the red-eared sliders from Nandujiang was
53.85%, Wanquanhe was 58.82% and Haikoudonghu
was 50.00%. The sample of group A from Nandujiang
carries 75.00% of
Salmonella
, and group B 50.00% of
Salmonella
(Table 2). 34 of red-eared sliders from
Wanquanhe in Group A carried 66.67% of
Salmonella
,
group B carried 56.00% of
Salmonella
, Whereas 8 of
red-eared sliders in group A carried 42.85% of
Salmonella
, group B was not for statistical analysis
due to small sample size.
Table 1 Biochemical test response to
Salmonella
of red-eared sliders
Types of biochemical
reactions
H
2
S
Indole
Urea
KCN
Lysine
decarboxlase
No of positive
individuls detected
A1
A1
-
1
+
-
-
+
24
A1
-
2
+
-
-
-
2
A1
-
3
+
-
+
+
32
A1
-
4
+
+
-
+
0
A2
A2
-
1
+
-
-
+
2
A3
A3
-
1
-
-
+/-
19
Note
：“
+
”
stands positive;
“-”
stands negative