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Molecular Pathogens
MP2011, Vol.2, No.1
http://mp.sophiapublisher.com
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Figure 2 A neighboring-joining tree based on 20 FMDV
complete genome sequences
Note: The 19 reference GenBank accession number are listed
as following: O/LZ, DQ248888; O/HKN/2002, AY317098;
O/ES/2001, AY686687; O Taiwan/97, AY593835; O strain
chu-pei, AF026168; O/Aksesu/58, AF511039; O/Tibet/CHA/99,
AJ539138; O/NY00, AY333431; O/Manisa/69, AY593823; O1
BFS/81/67, AY593815; Asia 1/IND 63/72, AY304994; Asia
1/JS/CHA/05, EF149009; C1/C-s8cl, AJ133357; C3/Arg/85,
AJ007347; A12 Valle strain 119, AY593752; A22 Turkey/65,
AY593765; SAT 1 BOT/1/68, AY593845; SAT2/KEN/57,
AY251473; SAT3/4 BEC 1/65, AY593853
200~211 were presented in Figure 5, in which 40~60
and 133~160 aa segments have a significant variation,
and C-terminal linear epitope 200~211 aa of VP1
protein showed much more conservative. The
133~160 aa of VP1 would facilitate GH loop structure,
which contains the highly conserved arginine-glycine-
aspartate (RGD) cell adhesion sites. Two leucine
residues located at positions +1 and +4 downstream of
the RGD were highly conserved. In addition to the
exquisitely specific RGD triplet, the residue at
position +2 were the only critical and specific
Figure 3 A neighboring-joining tree based on
VP1
nucleotide
sequences of FMDV serotype O
determinant within the loop in promoting cell
recognition of a viral ligand (Mateu et al., 1996).
Comparison of non-structural protein 3A showed that
a 10 aa deletion (position 93~102) in the 3A protein is
observed (Figure 6). A similar deletion exists in the
virus strain lineages of Cathay topotype (Taiwan/97, O
Chu-pei, O/HKN/2002 and O/LZ strain). The location
of this change is different from the portion of 3A
containing deletions in the chicken embryo-adapted
FMDVs (Giraudo et al., 1990) and Asian isolates of a
type O FMDV with the high virulence for swine
(Beard and Mason, 2000; Knowles et al., 2001; Núñez
et al., 2001), which might be an alternative mechanism
of host spectrum alteration.
2 Discussions
Sequencing and analysis of FMDV isolates for
studying virus structure and function would be one of
the very important way, because sequence differences