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Legume Genomics and Genetics (online), 2011, Vol. 2, No.3, 14-21
http://lgg.sophiapublisher.com
18
Table 2 Expression level of the T
1
generation transgenic
hsf8
plants derived from Hajiao5489 identified by real-time PCR
Sample
Primer
CT (dRn)
△
CT=
(CTtarget-CThskg)
△△
CT=
(
△
CTsample-
△
CTcalibrator)
2
-
△△
CT
Relative quantity (dRn)
H
2
O
H
2
O
hsf8
lectin
No CT
No CT
-
-
-
-
-
-
Hajiao5489
hsf8
lectin
27.55
28.79
-
1.24
-
0
-
1.000 00
-
T07
Ⅱ-
002
-
1
T07
Ⅱ-
002
-
1
hsf8
lectin
28.84
30.70
-
1.86
-
-
0.62
-
1.546 77
-
T07
Ⅱ-
002
-
2
T07
Ⅱ-
002
-
2
hsf8
lectin
34.02
29.65
4.37
-
5.61
-
0.020 57
-
T07
Ⅱ-
003
T07
Ⅱ-
003
hsf8
lectin
28.65
29.26
-
0.61
-
0.63
-
0.648 25
-
T07
Ⅱ-
010
-
1
T07
Ⅱ-
010
-
1
hsf8
lectin
29.27
28.46
0.81
-
1.84
-
0.280 86
-
T07
Ⅱ-
010
-
2
T07
Ⅱ-
010
-
2
hsf8
lectin
29.16
28.48
0.68
-
1.92
-
0.265 32
-
Figure 5
hsf8
expressions of individual T07-
Ⅰ
transgenic
plants of T
1
generation and non-transgenic plants Hajiao5337
by Real-time PCR analyses
Note: 1: Hajiao5337; 2: T07
Ⅰ
-003-1; 3: T07
Ⅰ
-003-2; 4: T07
Ⅰ
-005-1; 5: T07
Ⅰ
-002-2; 6: T07
Ⅰ
-006-1; 7: T07
Ⅰ
-006-2; 8: T07
Ⅰ
-006-3; 9: T07
Ⅰ
-007-1; 10: T07
Ⅰ
-007-2; 11: T07
Ⅰ
-007-3; 12:
T07
Ⅰ
-010-1; 13: T07
Ⅰ
-010-2; 14: T07
Ⅰ
-010-3; 15: T07
Ⅰ
-014;
16: T07
Ⅰ
-015-1; 17: T07
Ⅰ
-015-2; 18: T07
Ⅰ
-015-3
Figure 6
hsf8
expressions of individual T07
Ⅱ
transgenic
plants of T
1
generation and non-transgenic plants Hajiao5489
by Real-time PCR analyses
Note: 1: Hajiao5489; 2: T07
Ⅱ
-002-1; 3: T07
Ⅱ
-002-2; 4: T07
Ⅱ
-003; 5: T07
Ⅱ
-010-1; 6: T07
Ⅱ
-010-2
Many studies indicated that there were significant
differences in the capacities of
Agrobacterium
infection
in different plants. Therefore, the match
degree between the
Agrobacterium
and plant would be
very important in the research of genetic
transformation, in other words, ideal combination
between
Agrobacterium
and plants would directly
affect transformation efficiency. In this study, the two
kinds of
Agrobacterium
GV3101 and LBA4404 were
chosen for transformation of two soybean lines, both
of two strains had a strong ability to infect soybean
cotyledonary-node to generate much more resistant
buds. In comparison, the infective activity of strain
GV3101 was better than that of LBA4404. However,
it was difficult to be sterilization after co-culture due
to its high infective activity, which resulted in
acquiring resistant seedlings. We could not remove out
all
Agrobacterium
even if the concentration of
cephalosporin reached to 600 mg/L. It is obvious that
the growth of resistant buds will be influenced if
increasing the usage of antimicrobial. So choosing
antimicrobial is also important as choosing the active