Page 7 - Legume Genomics and Genetics

Legume Genomics and Genetics (online), 2011, Vol. 2, No.3, 14-21
http://lgg.sophiapublisher.com
17
Table 1 Expression level of the T1 generation transgenic hsf8 plants derived from Hajiao5337 identified by real-time PCR
Samples Primers CT (dRn) CT (dRn) △CT=
(CTtarget- CThskg)
△△CT=
(△CTample-△CTcalibrator)
2－△△CT
Relative quantity (dRn)
H2O
H2O
hsf8
lectin
No CT
No CT
No CT
No CT
-
-
-
-
-
-
Hajiao5337 hsf8
lectin
32.06
31.04
32.06
31.04
1.02
-
0.00
-
1.000 00
-
T07Ⅰ-003- 1
T07Ⅰ-003- 1
hsf8
lectin
34.30
34.69
34.30
34.69
- 0.39
-
-0.02
-
1.017 10
-
T07Ⅰ-003- 2
T07Ⅰ-003- 2
hsf8
lectin
28.40
27.86
28.40
27.86
0.54
-
-0.48
-
1.389 01
-
T07Ⅰ-005- 1
T07Ⅰ-005- 1
hsf8
lectin
29.68
33.28
29.68
33.28
- 3.60
-
-4.62
-
24.384 56
-
T07Ⅰ-005- 2
T07Ⅰ-005- 2
hsf8
lectin
27.04
27.48
27.04
27.48
- 0.44
-
-1.46
-
2.743 13
-
T07Ⅰ-006- 1
T07Ⅰ-006- 1
hsf8
lectin
30.43
29.65
30.43
29.65
0.78
-
-0.24
-
1.179 66
-
T07Ⅰ-006- 2
T07Ⅰ-006- 2
hsf8
lectin
26.35
28.21
26.35
28.21
- 1.86
-
-2.88
-
7.298 77
-
T07Ⅰ-006- 3
T07Ⅰ-006- 3
hsf8
lectin
36.16
31.98
36.16
31.98
4.18
-
4.55
-
0.042 50
-
T07Ⅰ-007- 1
T07Ⅰ-007- 1
hsf8
lectin
29.69
30.33
29.69
30.33
- 0.64
-
-1.66
-
3.146 02
-
T07Ⅰ-007- 2
T07Ⅰ-007- 2
hsf8
lectin
25.50
28.56
25.50
28.56
- 3.06
-
-4.08
-
16.861 91
-
T07Ⅰ-007- 3
T07Ⅰ-007- 3
hsf8
lectin
30.26
29.31
30.26
29.31
0.95
-
1.32
-
0.400 69
-
T07Ⅰ-010- 1
T07Ⅰ-010- 1
hsf8
lectin
32.58
30.36
32.58
30.36
2.22
-
2.59
-
0.165 97
-
T07Ⅰ-010- 2
T07Ⅰ-010- 2
hsf8
lectin
32.91
35.54
32.91
35.54
- 2.63
-
-2.26
-
4.793 46
-
T07Ⅰ-010- 3
T07Ⅰ-010- 3
hsf8
lectin
27.43
29.15
27.43
29.15
- 1.72
-
-2.74
-
6.670 27
-
T07Ⅰ-014
T07Ⅰ-014
hsf8
lectin
25.97
30.09
25.97
30.09
- 4.12
-
-5.14
-
35.217 15
-
T07Ⅰ-015- 1
T07Ⅰ-015- 1
hsf8
lectin
27.93
29.50
27.93
29.50
- 1.57
-
-2.59
-
5.996 08
-
T07Ⅰ-015- 2
T07Ⅰ-015- 2
hsf8
lectin
28.04
30.97
28.04
30.97
- 2.93
-
-3.95
-
15.308 50
-
T07Ⅰ-015- 3
T07Ⅰ-015- 3
hsf8
lectin
26.78
30.20
26.78
30.20
- 3.42
-
-4.44
-
21.587 62
-