Basic HTML Version
Legume Genomics and Genetics (online), 2010, Vol. 1, No.7, 34-40
http://lgg.sophiapublisher.com
35
M
1
generation by treatment of two different concentra-
tions had obviously differences in seedling rate, plant
survival rate and variation frequency. The greater the
treatment concentration was, the smaller the seedling
rate and plant survival rate would be, while the higher
the variation frequency would be, which might be caused
by the relatively great damages of high concentrations
on seeds (Table 1).
The plant height of M
1
generation was shown the greater
the treated concentration was, the shorter the plant height
would be. The 0.2% colchicine had the best treatment
effect by integrative analysis on node and branch number
on main stem, pod number per plant, yield per plant and
100
-
seed weight (Table 2).
The agronomic traits of mutants in M
2
and M
3
generation
were investigated, and the plant heights were similar to
those in the control (Table 3; Table 4), but these mutants
were superior to the control in pod number per plant,
the number of seeds per plant, yield per plant and 100
-
seed weight. The seed number per plant and 100
-
seed
weight of colchicine-induced M
2
generation exhibit sign-
ificant difference at 5% and 1% levels than the control’s,
respectively (Table 3). Only difference of 100
-
seed
Table 1 Percentage of plants and mutants of M
1
treated by different concentrations of colchicine
Treatment (12 h)
M
1
Seedling rate (%)
M
1
plant survival rate (%)
M
1
mutant frequency (%)
0.4% concentrations of colchicines 6.00cC
3.50cC
1.59aA
0.2% concentrations of colchicines 30.80bB
26.70bB
1.24bB
Control
92.00aA
89.00aA
0.00cC
Note: Different lowercases indicate significant (p<0.05), and with different capital letters are extra significantly different (p<0.01)
Table 2 The agronomic traits of M
1
by different concentrations of colchicine
Treatment (12 h)
0.4% colchicine
0.2% colchicine
Control
Plant height (cm)
28.77bA
35.80abA
43.93aA
No. of nodes on mainstem
8.13bAB
11.06aA
7.40bB
Starting nod of effective branch
0.53aA
1.44aA
1.70aA
Branch number
1.04bA
3.47aA
2.27abA
Pod length (cm)
6.04bA
6.25abA
7.07aA
Pod width (cm)
0.50bA
0.53abA
0.55aA
No. of pods per plant
5.12bB
13.14aA
7.27bB
No. of seeds per plant
15.81bB
32.10aA
27.47bB
Yield per plant
7.29bB
7.61aA
6.85cC
100
-
seed weight (g)
10.86cC
12.72aA
11.33bB
Note: Different lowercases indicate significant (p<0.05), and with different capital letters are extra significantly different (p<0.01)
Table 3 The Agronomic traits of M
2
treated by different concentrations of colchicine
Treatment (12 h)
0.4% colchicine
0.2% colchicine
Control
Plant height (cm)
21.53aA
22.67aA
20.97aA
No. of nodes on mainstem
11.39abA
12.56aA
10.74bA
Starting nod of effective branch
0.69aA
0.52aA
0.60aA
Branch number
0.23aA
0.16aA
0.19aA
Pod length (cm)
5.74aA
5.79aA
5.34aA
Pod width (cm)
0.59aA
0.61aA
0.57aA
No. of pods per plant
4.38aA
4.35aA
2.08aA
No. of seeds per plant
17.05aA
15.02aA
6.19bA
Yield per plant
2.06abA
2.76aA
0.56bA
100
-
seed weight (g)
12.46bA
13.80aA
9.36cB
Note: Different lowercases indicate significant (p<0.05), and with different capital letters are extra significantly different (p<0.01)