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Legume Genomics and Genetics (online), 2010, Vol. 1, No.7, 34-40
http://lgg.sophiapublisher.com
Research Article Open Access
Colchicine-induced Trait Variations in Azuki Bean (
Vigna angularis
)
Baomei Wu , Hongxia Liu , Bo Zhao , Xing Tong , Qingming Zhao , Ping Wan
Beijing University of Agriculture, College of Plant Science and Technology, Beijing, 102206
corresponding author email: pingwan3@yahoo.com.cn;
Authors
Legume Genomics and Genetics 2010, Vol.1 No.7 DOI:10.5376/lgg.2010.01.0007
Received: 30 Aug., 2010
Accepted: 28 Sep., 2010
Published: 20 Oct., 2010
This article was first published in the Molecular Plant Breeding (Regular Print Version), and here was authorized to redistribute under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article as:
Wu et al., 2009, Colchicine-induced Variations in Characters of Adzuki Bean, Molecular Plant Breeding, 7(6): 1169-1175 (DOI: 10.3969/mpb.007.001169)
Abstract
Seeds of Jinnong 6 variety of azuki bean were soaked in 0.2% and 0.4% concentration of colchicines for 12 hours,
respectively. The plant survival rates in M
1
generation were 26.7% and 3.5%, and the variation rates were 1.24% and 1.59%, by
which it was demonstrated that the higher the concentrations of colchicines, the lower the plant survival rate, and the greater the
variation frequence. The treatment of the 0.2% concentration of colchicines for 12 h was optimal for the mutation of azuki bean,
while more variation types were generated in the treatment of the 0.4% concentration. Compared with the control group, average
number of seed per plant and 100-seed weight of between control and M
2
generation with colchicine treatment showed significant
difference at 5% and 1% levels, respectively; 100-seed weight of M
3
generation had significant difference at 1% level. Three
excellent lines with dominantly higher yield were gained. 263 mutants of leaf color, leaf shape, stem twining, more branch number,
black pod, yellow-white seed, light red and deep seed color, large seed and late maturing in M
3
generation were obtained. The
variations of leaf shape involved sword, small heart-shaped leaf and kidney leaves. The variations of leaf color included dark green,
light green, yellowing and yellow spot. Early-maturing mutants were earlier from 10 to 15 days and late-maturing mutants were later
from 7 to 10 days than the control. The traits of sprawl, yellow-white seed, higher plant mutant can be steadily inherited in M
4
. The
traits of kidney leaf , small heart-shape, sword leaf mutants are mostly inherited, Some mutants of glave kidney leaf in M
3
separated
sword, kidney, heart-shape leaves in M
4
.
Keywords
Azuki Bean (
Vigna angularisi
(Willd.) Ohwi & Ohashi); Colchicine; Inducing-mutation; Mutant
Background
Mutation breeding is a way to induce crop mutation by
using physical and chemical factors artificially, and
breed fine varieties directly applying to production or
create new germplasm resources to improve existing
varieties based on the selection and identification of
mutants (Zhang et al., 1996). As the fundamental source
of genetic variation, mutation is of great significance
to research on genetics, breeding and evolution. Colch-
icine is a good polyploid mutagen and usually used for
chromosome doubling. It can also induce genetic variation
of biological traits besides doubling chromosome in
cells (Tiwarl and Khanorkar, 1984; Liu et al., 2006).
Azuki bean is one of cultivated species, classified to
Vigna of
Phaseoleae
in
Papilionaeeae
of Leguminosae.
It is also one of Chinese most ancient cultivated crops.
The seeds of Azuki bean are nutritious with high protein
and low fat, and also have medicinal values. Azuki bean
is good for health and a bean crop of Chinese traditional
foreign exchange earnings (Yu, 2005). Colchicine had
been used to induce allelic mutation in gramineae and
vegetables, resulting in the generation of variants early
maturing, multi-flowers, big leaves, multi-tillers and
multi-chloroplasts (Cai et al., 1992; Zhang et al., 1996;
2000; Caroline et al., 2003; Wang, 2008). However, few
studies are reported on mutation of bean plants induced
by colchicine. In this research, a number of mutants
inducing by colchicine were obtained, some excellent
variation plants were selected. It provided valuable basic
materials for the gene mapping and cloning of azuki
bean, studies on functional genomics, innovation of
germplasm and mutation breeding.
1 Results
1.1 Induced-mutation effect and influences on agro-
nomic traits
Colchicine-induced azuki bean M
1
had rather low seed-
ling rate and plant survival rate compared with the
control group, and its relative values were lower than
50%. The greatest change in colchicine treatment was
the plant height of M
1
significantly lower compared
with the control, while colchicine-treated yield factors
of M
2
and M
3
generation were increased than the control.
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