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Legume Genomics and Genetics (online), 2010, Vol. 1, No.3, 11-17
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16
cording to the
AhKAS
Ⅰ
cDNA (qKAS
Ⅰ
-F and qKAS
Ⅰ
-R) and β-actin (qActin-F and qActin-R) sequences.
The real-time RT-PCR reactions were performed by
using the SYBR Premix Ex
Taq
polymerase (TaKaRa,
Japan) according to the manufacturer’s instructions.
The expression of the gene was calculated relative to
the calibration sample and the β-actin to normalize the
sample input amount. All the experiments were per-
formed in triplicate to ensure the data accuracy.
Authors’contributions
Xiaoyuan Chi was responsible for the large part of data acquisition. Mingna
Chen, Qingli Yang, Ya’nan He, Lijuan Pan, Yuan Gao analyzed data and
wrote substantial parts of the paper. Shanlin Yu conceived the overall study,
performed the experiment designs and took part in the data analysis and the
writing. All authors read and approved the final manuscript.
Acknowledgements
This work was supported by a grant from Modern Agro-industry
Technology Research System (nycytx-19), National High-Tech Research
and Development Plan of China (2006AA10A114; 2007AA10Z189),
National Project of Scientific and Technical Supporting Program (2008
BAD97B04), the National Natural Science Foundation of China (31000728)
and the Natural Science Fund of Shangdong Province (ZR2009DQ004).
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