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Bioscience Methods
BM 2011, Vo.2, No.4, 21-30
http://bm.sophiapublisher.com
- 25 -
Table 1 Amino acid and monosaccharide compositions of purified
C. mosaicus
bell mucin
Amino acid
Compositiona (%)
Monosaccharide
Mole (%)
Serine
1.9
GalNAc
53.5
Arginine
0.3
GlcNAc
15.0
Glycine
0.5
Fructose
0.0
Aspartic acidb
0.5
Galactose
19.7
Glutamic acidb
10.0
Glucose
0.02
Threonine
24.8
Mannose
6.9
Alanine
22.9
Sialic acid
4.6
Proline
15.0
Lysine
0.3
Methionine
0.4
Valine
20.5
Isoleucine
1.7
Leucine
0.9
Phenylalanine
0.3
Note: a:
pmole mucin amino acid/total mucin amino acid×100%, where total mucin amino acids is the sum of mucin amino acids
(except for cysteine, tryptophan and tyrosine); b: asparagines and glutamine are recovered as their acid form: aspartic acid and
glutamic acid, respectively
Table 2 Effects of
C. mosaicus
mucins on the adhesion of
P. aeruginosa
(Paer 6294
-
GFP) to human corneal epithelial cells grown in culture
Protein
Final Concentration (μg /mL)
Number of adhering bacteria×10
5a
Inhibition
b
(%)
C. mosaicus
bell mucin
0.00
10.5 ± 0.5
-
25.00
4.2 ± 0.1
60.0
50.00
2.2 ± 0.2
79.0
100.00
1.4 ± 0.5
86.7
C. mosaicus
exudates mucin
0.00
10.5 ± 0.5
-
31.25
7.7 ± 2.0
26.0
62.50
6 ± 0.8
42.3
125.00
5.4 ± 1.2
48.1
250.00
4.6 ± 0.5
55.8
Bovine milk MUC1
0.00
6.5 ± 0.4
-
100.00
4.4 ± 0.4
32.3
BSA
0.00
6.5 ± 0.4
-
100.00
6.4 ± 0.7
1.5
Note: a: Mean number from two independent experiments (each with four replicates )±SE; b: [(number of adherent bacteria of
control-number of adherent bacteria of test sample)/number of adherent bacteria of control]×100%
analyses are consistent with a protein composed of
tandem repeats containing Val, Thr- GalNAc+ (+ is
indicative of a possible elongation of the carbohydrate
chain), Ala, Pro and Glu residues with an approximate