Basic HTML Version
Bioscience Methods
BM 2010, Vol.1, No.1
http://bm.sophiapublisher.com
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Figure 1 The polymorphism between two pairs of bulks in
12 individuals' genomic DNA
Note: A: RGC-derived 7A4L; B: RGC-derived
7A4(1407)/
Bfa
Ⅰ
; C: RGC-derived 4L17L/
Hin
d
Ⅲ
; D:
RGC-derived 4L17R/Cfo
Ⅰ
; R1,R2: Nematode-resistant
individuals in
‘
9145 family
’
; S1,S2: Nematode-susceptible
individuals in
‘
9145 family
’
; SW: Swingle citrumelo; LB:
LB6-2; M: 1 kb DNA ladder
groups in Figure 3, the genetic distance between the
two resistance genes' loci is about 12.1 cM in ‘9145
family’ and 17.2 cM in ‘9401 family
’
. The marker
Y65F+R/
Rsa
Ⅰ
developed from the
Ctv
resistance
candidate sequence was the only one located
between the
Ctv-Tyr1
regions in
‘
9401
’
linkage
map, but there are much markers located between
the
Ctv-Tyr1
regions in
‘
9145
’
linkage map.
2 Discussion
2.1 The bulked segregant analysis in citrus
genetics
The bulked segregant analysis has been successfully
and widely applied in much field of research,
especially in genetic markers selection and other
difference discriminating analysis (Mansur et al.,
1993), and has accelerated the mapping of many
genes in different plant species (Mohan et al., 1997).
Figure 2 Genetics linkage map of the nematode resistance
loci “
Tyr1
” (Deng et al., 2000)
Note: A and B were linkage maps constructed before and be
used here for referendum and comparing; C: The first round
building of linkage map of the nematode resistance loci
“
Tyr1
” with the previously obtained multiple markers and
the newly developed markers in the ‘9145 family’,
‘LB6
-
2’× ‘Swingle’BC1 population
Figure 3 Genetic linkage map of molecular markers in ‘9145
family’(A) and ‘9401 family’ (B)
Note: The specific markers 7A4(1407) and 7A4(2168) were
incorporated in the second round map construction of ‘9145
family’
Several genes in citrus for controlling important
horticultural traits, such as CTV resistance (Gmitter
et al., 1996; Deng et al., 1997), CN resistance
(Deng et al., 2000; Ling et al., 2000), dwarfing
(Cheng and Roose, 1995), and fruit acidity (Fang